1) Polymerase chain reaction
聚合酶链反应
1.
Application of adenosine deaminase in cerebrospinal fluid combined with real-time quantitative polymerase chain reaction in the diagnosis of elderly tuberculous meningitis;
脑脊液腺苷脱氨酶联合实时荧光定量聚合酶链反应在老年结核性脑膜炎诊断中的应用
2.
Application of polymerase chain reaction to assess genic polymorphism of oral Actinomyces;
聚合酶链反应在口腔放线菌基因多态性分析中的应用
3.
Clinical application of polymerase chain reaction assay in measurement of mycoplasma pneumoniae in children patients;
聚合酶链反应法在测定患儿肺炎支原体中的临床应用
2) PCR
聚合酶链反应
1.
Detection of tyrosine decarboxylase gene of lactic acid bacteria by PCR;
聚合酶链反应检测乳酸菌酪氨酸脱羧酶基因
2.
EXPERIMENTAL STUDY ON DETECTION OF EB VIRUS IN THE STD OUTPATIENTS WITH PCR;
聚合酶链反应检测门诊STD患者泌尿生殖道EB病毒感染的实验研究
3.
Cinical Significance of Detection of HCV Infection with FQ-PCR;
荧光定量聚合酶链反应检测丙型肝炎病毒的临床应用
3) polymerase chain reaction(PCR)
聚合酶链反应
1.
Methods: Genitourinary tract secretions were measured using polymerase chain reaction(PCR) methods and used two different primers detecting at the same time.
目的:建立聚合酶链反应技术(PCR)检测天津医科大学总医院性病门诊患者的分泌物标本中的Mg-DNA,以了解生殖支原体感染在此性病门诊患者中的发病情况。
2.
Objective:To measure Bactericides forsythus(Bf) in subgingival plaque samples of patients with chronic periodontitis by polymerase chain reaction(PCR) and to explore the correlation between the level of Bf and periodontal status.
目的:利用聚合酶链反应(PCR)检测吸烟与非吸烟者龈下福赛类杆菌(Bf)水平,通过Bf的检出率进一步探讨吸烟与牙周炎的关系。
3.
Methods To use polymerase chain reaction(PCR)and the restriction fragments length polymorphism(RFLP),type specific primers PCR,sequencing and sequence analysis method differentially.
方法分别进行聚合酶链反应及限制性片段长度多态性分析、型特异引物PCR分型、基因测序及序列分析。
4) polymerase chain reaction (PCR)
聚合酶链反应
1.
The value of polymerase chain reaction (PCR) for the diagnosis of gonorrhea urethritis;
聚合酶链反应技术在淋菌性尿道炎诊断中的价值
2.
Methods Three kinds of special sequence primers (SSP) for polymerase chain reaction (PCR) with regard to the mutation styles (CGT, GAT and GGT) at codon 12 of K ras were used to study the human pancreatic carcinoma cell line PC 2.
方法 针对K ras基因第 1 2位密码子点突变方式 (CGT、CAT、GTT)设计顺序特异性引物 (SSP) ,对人胰腺癌细胞株PC 2进行聚合酶链反应 (PCR) ,扩增产物借助聚丙烯酰胺凝胶电泳判定该细胞株有无K ras基因点突变及其突变方式。
3.
Polymerase chain reaction (PCR) is a method for the primer -directed enzymatic amplification of specific DNA sequences in vitro and continuation of PCR for a few hours may result in 10~7-10~8times amplification of DNA region of interest.
聚合酶链反应(PCR)是一种体外扩增目的基因片段技术，这种技术可在几小时内将目的基因片段扩增10~7-10~8倍。
5) RT-PCR
聚合酶链反应
1.
Methods In 40 patients with ovarian cancers,TFPI-2 mRNA was detected at center and periphery of tumor and at nearby normal tissue by RT-PCR.
方法以β-actin基因为参照,应用逆转录聚合酶链反应(RT-PCR)技术,检测40例卵巢癌患者的肿瘤中心处癌组织、肿瘤边缘癌组织及癌旁正常卵巢组织中TFPI-2的表达。
2.
[Method] The gene expression of Th1 and Th2 cytokines before and after chemotherapy in breast cancer was detected by reverse transcription polymerase chain reaction(RT-PCR).
[方法]采用逆转录聚合酶链反应(RT-PCR)技术,检测乳腺癌患者化疗前后外周血单个核细胞(PBMC)Th1和Th2类细胞因子mRNA表达水平的变化。
3.
Methods The expression of PRAME mRNA was detected in 36 CML, 20 lymphoma, 20 MM, 10 normal subjects and one AL cell-line(K562) by using semi-quantitative RT-PCR and compared with the expression of BCR/ABL in 36 CML.
方法：应用半定量逆转录-聚合酶链反应(RT-PCR)技术，检测76例血液肿瘤病人，其中36例慢性粒细胞白血病(CML)、20例淋巴瘤(NHL18例，HD2例)、20例多发性骨髓瘤(MM)，10例正常人骨髓单个核细胞(BMMNC)以及K562细胞株PRAME mRNA的表达水平；同时检测CML患者BCR／ABL融合基因的表达并与PRAME的表达结果相比较；并跟踪观察1例MM患者骨髓PRAME基因的表达情况。
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条