1) DGGE
变性梯度凝胶电泳
1.
Nested PCR and DGGE were used to amplify and separate 16S rDNA of α-Proteobacteria and β-Proteobacteria.
采用PCR-DGGE等分子生物学技术,从平行AN/AO工艺六个反应池的活性污泥样品中提取总DNA,采用套式PCR(nested PCR)进行两轮PCR扩增,对α-Proteobacteria、β-Proteobacteria的16S rDNA片段进行DGGE(变性梯度凝胶电泳)分离,从而分析活性污泥样品中该类除磷菌的种群结构。
2.
The molecule biological techniques DGGE, clone and real-time PCR were utilized to study prinimilarily the microorganism in 2 anaerobic ammonia oxidation reactors.
利用变性梯度凝胶电泳、克隆和实时PCR等分子生物学技术对2个厌氧氨氧化反应器中的微生物进行了初步研究。
3.
The acidophiles composition and community structure in the sediment was studied with 16S rDNA clone library and denaturing gradient gel electrophoresis(DGGE).
采集了酸性矿山废水区域酸性沉积物样品,运用16S rDNA克隆文库技术和变性梯度凝胶电泳(DGGE)技术,研究了沉积物中的嗜酸菌类群以及它们的群落结构。
2) denaturing gradient gel electrophoresis
变性梯度凝胶电泳
1.
Analysis of mutant frequency of PCR cloning by denaturing gradient gel electrophoresis;
用变性梯度凝胶电泳分析PCR克隆的突变率
2.
Detection of denaturing gradient gel electrophoresis in pathogenic bacteria of periodontitis
牙周炎致病菌的变性梯度凝胶电泳检测
3.
Evaluation of oral microbial diversity with denaturing gradient gel electrophoresis
变性梯度凝胶电泳分析口腔微生物多样性的研究进展
3) denaturing gradient gel electrophoresis(DGGE)
变性梯度凝胶电泳
1.
Protocols of applification of denaturing gradient gel electrophoresis(DGGE) in studies of environmental microorganism;
变性梯度凝胶电泳在环境微生物研究中的应用详解
2.
For an analysis of microbial diversity in the A2/O process,the polymerase chain reaction(PCR) and denaturing gradient gel electrophoresis(DGGE) techniques were applied.
通过PCR-DGGE技术对A2/O工艺中的微生物多样性进行了分析,以细菌和古细菌16s rRNA基因通用引物530F/1490R对A2/O活性污泥中提取的细菌基因组DNA进行PCR扩增,扩增产物经纯化后用于变性梯度凝胶电泳(DGGE)分析。
3.
With the oil-polluted soils after converted from paddy field to upland field for different years in the Shenfu wastewater irrigation area of Shenyang as test materials,the concentrations of their oil pollutants were determined,and the microbial community structure was investigated by denaturing gradient gel electrophoresis(DGGE) and phospholipids fatty acid(PLFA) methods.
对沈抚灌区水改旱田不同年限土壤的石油污染物浓度及组分进行了分析,并采用变性梯度凝胶电泳和磷脂脂肪酸分析方法,分析了污染土壤微生物群落结构的变化。
4) denaturing gradient gel electrophoresis (DGGE)
变性梯度凝胶电泳
1.
Adopting 16S rDNA of denaturing gradient gel electrophoresis (DGGE) technology to reveal nitrifying bacteria community (ammonium oxidizing bacteria and nitrite oxidizing bacteria) denitrogenation action in constructed rapid infiltrition system (CRI).
采用16SrDNA的变性梯度凝胶电泳(DGGE)技术研究人工快速渗滤系统(CRI)中硝化菌群(氨氧化菌和亚硝酸氧化菌)的空间分布规律。
2.
After purifying the genomic DNA, 16S rRNA genes were amplified, and the amplified DNA fragments were separated by denaturing gradient gel electrophoresis (DGGE).
为了研究生物造粒流化床污水处理反应器颗粒污泥的微生物种群多样性,分别从生物造粒流化床10、60和110cm处取颗粒污泥,通过细胞裂解直接提取颗粒污泥细菌基因组DNA,PCR扩增后经变性梯度凝胶电泳(DGGE)分离,获得微生物群落的DNA特征指纹图谱,对特征条带进行序列测定及序列同源性分析。
5) denaturing/temprature gradient gel electrophoresis
变性温度梯度凝胶电泳
补充资料：Sds-聚丙烯酰胺凝胶电泳凝胶电泳
sds-聚丙烯酰胺凝胶电泳（sds-page，sodium dodecyl sulfate-polyacrylamide gel electrohoresis）
在有去污剂十二烷基硫酸钠存在下的聚丙烯酰胺凝胶电泳。sds-page只是按照分子大小分离的，而不是根据分子所带的电荷和大小分离的。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条