1) quantitative PCR
定量PCR
1.
Quantitative PCR Techniques and Its Application Status;
定量PCR技术及其应用现状
2.
A quantitative PCR kit used in automatic genetic amplification instrument;
全自动(定量PCR)基因扩增诊断仪配套定量试剂的研制
3.
Real-time fluorescence quantitative PCR and its application in animal nutrition;
荧光定量PCR技术及其在动物营养中的应用
2) real-time PCR
定量PCR
1.
Expression analysis of Notch receptors in peripheral blood mononuclear cells of patients with rheumatoid arthritis by real-time PCR;
定量PCR检测类风湿关节炎患者外周血单个核细胞Notch基因表达
2.
Quantitative Study of Porphyromonas Gingivalis by Real-time PCR of Orthodontic Patients with Fixed Orthodontic Appliances;
定量PCR检测正畸治疗前后牙龈卟啉单胞菌的变化
3.
Samples were sampled from the influent,effluent and supernatant fluid in each MBR and were detected by a Taqman-based real-time PCR technology.
研究了不同孔径的膜生物反应器对游离白斑综合症病毒(white spot syndrome virus,WSSV)的截留效果,对不同膜生物反应器的进水、上清液、出水进行荧光定量PCR检测。
3) real time PCR
定量PCR
1.
Comparison of three protocols for extraction of total fecal DNA and identification of Salmonella with real time PCR
粪便基因组DNA提取方法比较及在沙门菌定量PCR检测中的应用
2.
Identification of Salmonella in lobster sauce with real time PCR
绝对定量PCR快速检测豆豉沙门菌
3.
Methods The amount of COX-2 mRNA of HT 29 cells,which were cultured in DMEM containing the concentration of 250,500 and 1?000?nmol/L Se for 4,8 and 24?h,was detected by real time PCR.
方法 选用人结肠癌细胞系HT - 2 9,采用定量PCR方法测定不同剂量 (0 2 5 ,0 5 ,1 0 μmol/L)硒在 4 ,8和 2 4h对COX - 2mRNA表达的影响。
4) Real-time quantitative PCR
定量PCR
1.
Real-time quantitative PCR detection of the Barnase gene in genetically modified phosphinothricin-tolerant canola;
转基因抗草丁膦油菜籽中Barnase基因的实时荧光定量PCR检测
2.
The expressions of Zfx were measured by real-time quantitative PCR in mRNA level and by western blot and immunohistochemisty in protein level.
方法:收集52例经病理证实的脑胶质瘤肿瘤标本和8例正常脑组织标本,根据病理结果对脑胶质瘤标本进行分组,通过实时定量PCR、免疫组织化学和Westernblot等方法对标本中Zfx的mRNA和蛋白表达进行检测,应用统计学方法对正常脑组织和不同恶性程度脑胶质瘤中Zfx的表达进行分析。
5) quantitative RT-PCR
定量RT-PCR
1.
Quantitative RT-PCR in detecting the apoptosis associated gene expression in penumbra area of cerebral infarction in rats;
用定量RT-PCR方法检测脑梗死半影区内凋亡相关基因的表达
2.
Using Immunomagnetic Beads and Quantitative RT-PCR for Detecting Micrometastasis in Peripheral Blood among Patients with Gastric Cancer;
联用免疫磁珠及定量RT-PCR检测胃癌外周血微转移
3.
Objective The purpose of this study is to determine the expression level of CK19 in sentinel lymph nodes(SLN)of breast cancer by quantitative RT-PCR,and to improve the detection rate of micrometastasis in breast cancer.
目的探讨定量RT-PCR法检测细胞角蛋白(CK19)在乳腺癌前哨淋巴结(SLN)中的表达,提高前哨淋巴结活检中微转移的检出率。
6) Semi-quantitative PCR
半定量PCR
1.
Technology and application of cariogenic streptococcus mutans detection by semi-quantitative PCR;
半定量PCR检测致龋性变形链球菌的技术及应用
2.
A semi-quantitative PCR was developed and used to demonstrate the reverse transcriptase activity of the recombinant protein.
通过一种半定量PCR方法,检测到所表达的蛋白具有逆转录酶活性。
3.
Methods Semi-quantitative PCR method was used in this research to estimate PERV copy-number of four mini-pigs in China.
方法采用半定量PCR方法检测了我国四种小型猪内源性逆转录病毒基因的拷贝数。
补充资料：immune PCR
分子式：
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
CAS号：
性质： 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上，从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上，进而证明有抗原存在。目前最为敏感的检测方法，理论上可测得一个抗原分子的存在。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条