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1)  ELISA [英][i'laizə]  [美][ɪ'laɪzə]
酶联免疫吸附试验
1.
Application of ELISA for microcystins detection;
酶联免疫吸附试验在微囊藻毒素检测中的应用进展
2.
Effection of Sample Factors to ELISA Detection;
标本因素对酶联免疫吸附试验测定的影响
3.
Common Factors of Influencing HBsAg and Countermeasure in ELISA;
酶联免疫吸附试验影响HBsAg常见因素与对策
2)  Enzyme-linked immunosorbent assay
酶联免疫吸附试验
1.
Comparison of enzyme-linked immunosorbent assay and direct fluorescent antibody for the detection of Chlamydia trachomatis
酶联免疫吸附试验和直接免疫荧光检测沙眼衣原体的方法学比较
2.
[Methods] PG Ⅰ and Ⅱ were measured with the two-site "sandwich" technique of enzyme-linked immunosorbent assay(ELISA).
目的确定胃蛋白酶原PGⅠ、PGⅡ酶联免疫吸附试验(ELISA)试剂盒的分析灵敏度及其对胃癌的诊断价值。
3.
METHODS: Enzyme-linked immunosorbent assay was used to measure the levels of serum HBV-LP, HBV preS1, HBV preS2 and HBV markers, and fluorescence quantitative-polymerase chain reaction (FQ-PCR) was used to detect HBV DNA in 162 patients with hepatitis B as well as 47 normal controls.
方法:对162例HBV感染者YL47A正常对照血清采用酶联免疫吸附试验检测HBV-LP,HBV 前S1抗原,HBV前S2抗原及乙型肝炎血清标志物:FQ-PCR定量检测HBV DNA。
3)  enzyme linked immunosorbent assay
酶联免疫吸附试验
1.
Evaluation of enzyme linked immunosorbent assay for the detection of West Nile virus antibodies;
西尼罗病毒抗体酶联免疫吸附试验检测方法的评价
2.
A double sandwich enzyme linked immunosorbent assay utilizing linear sweep polarographic detection (ELISA-LSP) for the detection and type identification of herpes simplex virus (HSV) antigen was established.
酶联免疫吸附试验(ELISA)与单扫描示波极谱法(LSP)检测相联接,用自制的小型“DME-Pt-Ag/AgCl”三电极系统,与商品酶联板配套,直接检测酶促反应电活性产物,建立了ELISA-LSP法检测单纯疱疹病毒(HerpesSimplexVirus,HSV)抗原并进行分型的方法。
3.
Objective To explore the clinical value and relationship between time-resolved fluoroimmunoassay(TRFIA) to detect HBV M in quantity and enzyme linked immunosorbent assay(ELISA) to detect HBV M in quality.
目的探讨时间分辨荧光免疫测定法(TRFIA)定量检测乙型肝炎血清学标志物(HBVM)与酶联免疫吸附试验(ELISA)定性检测HBVM的关系及其临床意义。
4)  Enzyme-linked immunosorbent assay(ELISA)
酶联免疫吸附试验
1.
TTV-DNA and anti-TTV-IgG of the sera of the patients with hepatic diseases(132 cases) and the blood donors(120 cases) were detected simultaneously by polymerase chain reaction(PCR) and enzyme-linked immunosorbent assay(ELISA).
采用聚合酶链反应 (PCR)和酶联免疫吸附试验 (EL ISA) ,分别检测 132例各型肝病患者和 12 0例献血员血清 TTV- DNA和抗 TTV- Ig G。
5)  Enzyme-Linked Immunosorbent Assay (ELISA)
酶联免疫吸附试验
1.
The rabbit anti-human CIDE-3 antibody was analyzed by Western blot, enzyme-linked immunosorbent assay (ELISA) and immunohisto.
coli BL21(DE3),IPTG诱导表达CIDE-3蛋白,经Ni-NTA Agarose纯化后免疫新西兰白兔,获得人CIDE-3蛋白兔抗血清,并通过免疫印迹(Western blot)、酶联免疫吸附试验(ELISA)及免疫组织化学法对抗体进行鉴定。
2.
The content of EGFR and SBEM in peripheral blood were determined by enzyme-linked immunosorbent assay (ELISA).
采用酶联免疫吸附试验(Enzyme-Linked Immunosorbent Assay,ELISA)定量检测两组患者外周血中EGFR和SBEM的含量,并分析二者与乳腺癌临床病理参数的关系以及二者联合检测的意义。
3.
Methods Serum levels of endostatin were measured by an enzyme-linked immunosorbent assay (ELISA) in 44 patients with lung cancer,20 patients with benign lung diseases and 20 normal persons.
方法应用酶联免疫吸附试验(ELISA法),测定44例原发性肺癌、20例肺部良性疾病和20例正常人血清内皮抑素含量。
6)  ELISA [英][i'laizə]  [美][ɪ'laɪzə]
间接酶联免疫吸附试验
1.
The positive clones were picked out and identified by ELISA and WB.
方法以中国莱姆病螺旋体伽氏疏螺旋体(Borreliagarinii)的代表菌株PD91的全菌蛋白为抗原,免疫BALB/c小鼠,取脾细胞与骨髓瘤细胞SP2/0融合,用间接酶联免疫吸附试验(ELISA)和蛋白免疫印迹方法(WB)筛选,并经过2次或3次克隆,以获得单克隆抗体。
2.
Methods Serum IgM antibodies of influenza virus A and B(FluA and FluB),respiratory syncytial virus(RSV),adenovirus(ADV)and parainfluenza virus(PIV)were detected by indirect enzyme linked immunosorbent assay(ELISA).
方法采用间接酶联免疫吸附试验(ELISA)方法检测血清流感病毒A(FluA)、流感病毒B(FluB)、呼吸道合胞病毒(RSV)、腺病毒(ADV)和副流感病毒(PIV)的IgM抗体。
3.
leprae and their trends in sera from NC (192 cases) and CLP (666 cases, including 8 cases relapsed) in ELISAs using ND, Ms, LAM B and α 2 as antigens were mearsured.
方法 : 用间接酶联免疫吸附试验(ELISA)检测正常人 (192例 )和愈后原麻风病人 (6 6 6例 ,含 8例复发者 )血中抗体水平及其态势 ,计算各抗原检测结果间的个体符合率并加以比较。
补充资料:酶联免疫吸附试验


酶联免疫吸附试验


酶联免疫吸附试验是将可溶性抗原或抗体吸附(包被)于固相载体(目前常用微孔反应板)上,加入待检样品,所测未知抗体或抗原与包被的抗原或抗体结合,形成抗原抗体复合物,待依序加入酶结合物(酶标记抗体或酶标记抗原)和该酶的底物时,产生呈色反应。呈色程度与检样中待测抗体或抗原的量相关,可据此定性或定量。用于检测精液或宫颈黏液中的抗精子抗体,并行定量分析。方法是以精子或精子抗原提取物放入微试管,加入被测精液或宫颈黏液及抗免疫球蛋白—酶结合物,再加入酶底物观察颜色变化,以自动计算器进行定量测定。
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