1) luciferase
[英][lju:'sifə,reis] [美][lu'sɪfə,res, -,rez]
虫荧光素酶
1.
Study on the Trehalose Protection on Activity of Luciferase;
海藻糖对虫荧光素酶活性保护作用研究
2.
Objective To study the DTA vector containing the Ewing family of tumor s EWS-FLI1 binding sequence depresses the co-transferred luciferase reporter plasmid.
结果 每增加一个 pS2 DTA剂量梯度 ,在尤文肉瘤细胞中都可以检测到虫荧光素酶的表达显著降低 ,而在对照细胞中这种作用却不明显 ;在同一个pS2 DTA剂量梯度下 ,虫荧光素酶在尤文肉瘤细胞的表达都显著高于非尤文肉瘤细胞。
3.
Methods The transfection of luciferase reporter plasmid(pS2),which contains the EWS FLI1 binding sequence,into cultured Ewing′s sarcoma cell lines(RD ES,SK ES) and control cell lines (Hela,NIH3T3)was performed by liposome method and luciferase activity in the cellular lysate was measured by spectrofluorometer.
结果 虫荧光素酶在尤文肉瘤细胞中表达强度明显高于非尤文肉瘤细胞。
2) Firefly luciferase
虫荧光素酶
1.
Determination of concentration of ATP by immobilized firefly luciferase reactor;
固定化虫荧光素酶反应器测定 ATP 的初步研究
2.
To assess quickly the potential environmental hazard of heavy metal pollutant by the gene recombinant cell, a reporter vector regulated by electrophile response elements ( EPRE) was constructed by fusing EPRE to the TK minimal promoter and the gene encoding firefly luciferase (LUC) with biomolecular techniques.
研究用基因重组细胞快速评价重金属污染物对健康的危害,采用分子生物学方法,将亲电性化学反应元件(EPRE)与TK基本启动子和编码虫荧光素酶(LUC)基因相融合,构建成EPRE调控的LUC报告载体,将其转染于HeLa细胞。
3) firefly luciferase (FFL)
荧光虫荧光素酶
4) firefly luciferase
萤火虫荧光素酶
1.
The article researches the affect deletion of N terminal amino acids of firefly luciferase on activity of the enzyme.
由于N末端缺失6个氨基酸的突变体保持了77%的酶活性,因而萤火虫荧光素酶N末端第7个氨基酸与酶的催化活性密切相关。
2.
Objective: To study the agreement between chemiluminescence assay(Che) and bioluminescence imaging(Bio) used for detecting the firefly luciferase(Fluc) activity.
目的:比较2种萤火虫荧光素酶活性检测方法的一致性。
3.
In this research,the luc gene coding firefly luciferase(LUC) was amplified by using the DNA of Luciferase reporter vector pXP2 as the template.
以荧光虫荧光素酶报告载体pXP2 DNA为模板,扩增得到编码萤火虫荧光素酶(LUC)的基因(luc),构建了诱导型表达载体pQE30-luc。
5) Firefly luciferase gene
虫荧光素酶基因
补充资料:虫荧光素
分子式:
CAS号:
性质:从萤火虫(photinuspyralis)中提取而得的一种荧光素。淡黄结晶(难结晶)。熔点190℃(分解)。旋光度[α]D24-29°(DMF)。在荧光素酶的作用下,荧光素与ATP反应生成腺苷-荧光素,并释放焦磷酸。腺苷酰荧光素被认为是活性荧光素分子,它可被空气中的氧过氧化,形成腺苷酰氧化荧光素,随即分解为氧化型荧光素、水和腺苷酸,并伴有发光。
CAS号:
性质:从萤火虫(photinuspyralis)中提取而得的一种荧光素。淡黄结晶(难结晶)。熔点190℃(分解)。旋光度[α]D24-29°(DMF)。在荧光素酶的作用下,荧光素与ATP反应生成腺苷-荧光素,并释放焦磷酸。腺苷酰荧光素被认为是活性荧光素分子,它可被空气中的氧过氧化,形成腺苷酰氧化荧光素,随即分解为氧化型荧光素、水和腺苷酸,并伴有发光。
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