1) Pichia pastoris
毕赤酵母菌
1.
Purification of GLP-1/albumin Expressed in Pichia pastoris;
在毕赤酵母菌中表达的hGlp-1-白蛋白融合蛋白的纯化
2.
Secreted Expression of Signaling Protein 14-3-3 of Schistosoma japanicom in Pichia pastoris System with Primary Evaluation on its Antigenicity;
日本血吸虫信号蛋白14-3-3在毕赤酵母菌中的分泌表达及其抗原性分析
3.
Expression of anti-keratin human Fab in Pichia pastoris and optimization of expression condition;
人源性抗角蛋白抗体Fab段在巴氏毕赤酵母菌中的表达及表达条件的优化
2) Pichia pastoris
毕赤氏酵母菌
1.
SpreS1 has successfully expressed intracellular in Pichia pastoris,for the purpose of facilitated purification,6-Histidine-tag and 6-Histidine-EK-tag were separately introduced into 3 and 5 terminus of SpreS1-encoding DNA and the fusion genes were subcloned into the expression vector pPIC3.
5k-SS1-6His转化毕赤氏酵母菌GS115,筛选鉴定得到的重组酵母工程菌进行诱导培养,然后采用镍离子柱(Ni-NTABasin)纯化表达产物,纯化的蛋白样品经ELISA效价测定、SDS-PAGE和WesternBlot分析,结果表明融合抗原基因6His-EK-SS1和SS1-6His在毕赤氏酵母中均能表达,纯化的产物仍有免疫原性,所用的纯化方法简便有效。
2.
The recombinant plasmid was integreted into Pichia pastoris X 33 by electroporation and the expressed products were analyzed with SDS PAGE and Western blotting.
以 pPICZαB为载体 ,应用RT PCR从感染D2V的C6 / 36病变细胞中克隆全长E基因 ,电转化法将重组质粒整合入巴斯德毕赤氏酵母菌 ,经抗生素筛选、表型鉴定和PCR分析得到Mut+ 型的多拷贝整合菌 ,经甲醇诱导培养可产生 6 9kD的融合蛋白 ,与含组氨酸尾的D2V包膜糖蛋白分子量理论值相符 ;免疫印迹证实该表达产物可与D2VE特异性单抗和D2V多抗进行反应 ;表达产物经金属螯合亲和层析可获得纯化的含组氨酸尾的E融合蛋白并保留其免疫反应性。
3) Recombinant Pichia pastoris
毕赤酵母重组菌
1.
Application of Direct Gene Disruption Method in Recombinant Pichia pastoris;
毕赤酵母重组菌株直接基因缺失方法的研究
4) Pichia pastoris
毕赤酵母
1.
Feed-batch fermentation of Pichia pastoris engineering strain;
毕赤酵母工程菌产植酸酶补料分批发酵研究
2.
Effects of glycerol feeding strategies on production of recombinant reteplase from a methylotrophic yeast Pichia pastoris;
甘油相控制策略对重组毕赤酵母表达瑞替普酶(reteplase)的影响
3.
Artificial Neural Network-based Modeling of Biomass Concentration of Recombinant Yeast Pichia Pastoris at Expression Phase;
基于人工神经网络的重组毕赤酵母表达期菌体浓度模型
5) P.pastoris
毕赤酵母
1.
Expression of Soluble Recombinant Human Antithrombin Ⅲ(AT-Ⅲ) inP.pastoris;
人抗凝血酶Ⅲ(AT-Ⅲ)在毕赤酵母中可溶性表达
2.
Study on the transduction activity of Tat oligopeptide (I)——expression and purification of the model protein Tat-GFP in P.pastoris;
Tat短肽跨膜递送作用的研究(I)——模型蛋白Tat-GFP在毕赤酵母中的表达与纯化
3.
High Level Secretion Expression of Beefy Meaty Peptide Gene in P.pastoris;
牛肉风味强化肽(BMP)在毕赤酵母中的高效分泌表达
6) Pichia pastoris yeast
毕赤酵母
1.
Expression and identification of cecropin D gene cloned in Pichia pastoris yeast cells;
抗菌肽D基因在毕赤酵母中的表达及鉴定
2.
The extended DNA was assembled onto Pichia pastoris yeast X33 for expression.
设计并克隆了一个以源于蚕丝丝素蛋白结晶区的序列为GAGAGS(GlyAlaGlyAlaGlySer)的典型肽段为基本框架的基因,并导入毕赤酵母(Pichiapastoris)X33内进行了表达。
补充资料：毕陵
【毕陵】
（人名）毗陵伽婆蹉之略。比丘名。
（人名）毗陵伽婆蹉之略。比丘名。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条