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1)  GL-7-ACA acylase
GL-7-ACA酰化酶
1.
7β (4-Carboxybutanamido) cephalosporanic acid acylase (GL-7-ACA acylase) was purified from cell-free extracts of a recombinant, CU334 by a procedure invoiving ammonium suifate fractionation and successive column chromatography on DEAE-Sephadex A-50, DEAE-cellulose DE-52, Sephadex G-200 and Hydroxylapatide with 22-fold purification, 4.
CU334是高表达GL-7-ACA酰化酶工程菌,其菌悬液用超声波处理后,经硫酸铵分级沉淀、DEAE-Sephadex A-50离子交换柱层析、DEAE—纤维素DE-52柱层析、Sephadex G-200凝胶过滤及羟基磷灰石吸附柱层析等步骤,得到了凝胶电泳均一的GL-7-ACA酰化酶蛋白,纯化了22倍,得率4。
2.
This paper has determined the K_m and V_(max) of native immobilized GL-7-ACA acylase and free enzymes.
通过对国产固定化GL-7-ACA酰化酶和游离酶的表观米氏常数(Km)及最大反应速度(Vm)的测定。
3.
By taking advantage of uniform design and using national materials, the formula of fermentation media of GL-7-ACA acylase was optimized and gained better effect.
采用国产原料,应用均匀设计优选试验方法,对GL-7-ACA酰化酶生产用的发酵培养基配方进行了优化,取得了良好的效果,最终摇瓶效价达3919。
2)  Immobilized GL-7-ACA Acylase
固定化GL-7-ACA酰化酶
1.
Preparing D-7-ACA with tri-enzyme method(Immobilized D-amino acid oxidase,Immobilized GL-7-ACA Acylase,Immobilized deacetyl esterase) is an original,sustainable and brand new technology in promoting the development of green pharmaceutical industry in our country.
三酶(D-氨基酸氧化酶、固定化GL-7-ACA酰化酶和固定化去乙酰基酯酶)法制备D-7-ACA工艺,属国内首创的新技术、新工艺,有利于我国绿色制药工业的发展,是一种可持续的工艺路线。
3)  immobilized glutaryl-7-ACA acylase (GA)
固定化戊二酰-7-ACA基酰化酶(GA)
4)  Glutaryl-7-ACA
戊二酰基-7-氨基头孢烷酸(Gl-7-ACA)
5)  Glutaryl-7-aminocephalosporanic acid (Gl-7-ACA)
戊二酰基-7-氨基头孢烯酸(Gl-7-ACA)
6)  GL-7ACA acylase
GL-7ACA酰化酶
1.
Glutaryl-7-aminocephalosporanic acid acylase(GL-7ACA acylase) is one of important industrial enzymes for the production of 7-aminocephalosporanic acid(7-ACA),in this paper,a new metal chelating carrier(EIPARG-IDA-Co2+)was prepared to immobilize GL-7ACA acylase.
采用金属鳌合亲和层析技术,以EIP-ARG-IDA-Co2+为载体,从可溶性总蛋白中一步分离纯化具有His-tag标签的GL-7ACA酰化酶。
补充资料:Basacryl Yellow X-3GL
分子式:C21H25N2O·ZnCl3
分子量:508.92
CAS号:4208-80-4

性质:带绿光的黄色粉状,微溶于水冷水,较易溶于热水,也溶于酒精,均呈黄色。在浓硫酸中呈红光淡黄色,稀释后转绿光黄色。染色时,遇铜色泽微有变化,遇铁和铬色泽不变。

制备方法:由1,3,3-三甲基吲哚啉乙醛与2,5-双甲氧基苯胺经缩合、成盐、过滤及干燥即得成品。原料消耗(kg/t)1,3,3-三甲基吲哚啉乙醛 4852,5-双甲氧基苯胺 370

用途:用于腈纶的染色和印花。加助剂可用于改性涤纶和乙酸纤维的染色,还可染纸张。适用在腈纶、改性涤纶和乙酸纤维织物上直接印花。染腈纶呈艳黄色,主要用于染腈纶散纤维、纤维条和绒线。除单独染艳黄色外,常与阳离子红GTL或红BL拼染鲜艳的橙至大红色。

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