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1)  whole cell PCR
全细胞PCR法
1.
In this paper,three new detection methods,whole cell PCR,DNA prole and reporter gene cell receptor are discussed with the aim of detecting the minute amount of red tide toxin and harmful organism to prevent them from enteri.
对利用分子生物学技术对赤潮毒素进行检测的几种方法———全细胞PCR法、DNA探针法及信使基因细胞受体法进行了讨论。
2)  whole-cell PCR
全细胞PCR
1.
Investigation of algae pollution in Xiliu Lake and identification of toxic cyanobacteria by whole-cell PCR;
西流湖水体藻类污染现状和产毒蓝藻的全细胞PCR检测
3)  Single cell PCR
单细胞PCR
1.
The single cell PCR method, which has been developed recently, has many advantages in HAB studies, especially for those species which are difficult to culture in the laboratory.
其中,单细胞PCR方法是对难以室内培养的有害赤潮藻种进行遗传特征研究的一项重要技术。
4)  in cell PCR
细胞内PCR
5)  single-cell RT-PCR
单细胞RT-PCR
1.
Methods: Human neutrophils were isolated and purified from volunteers,total RNA was extracted and a regular RT-PCR aiming at IL-8Rβ mRNA was performed to ascertain its expression profile in human neutrophils and optimize the reaction conditions for the following single-cell RT-PCR procedures.
方法:采用倍比稀释相对定量单细胞RT-PCR方法分离纯化人外周血中性粒细胞并提取总RNA,针对IL-8RβmRNA设计引物,进行RT-PCR确认IL-8Rβ在中性粒细胞中的基因表达并优化反应条件。
6)  in-cell reverse transcription poly-merase chain reaction(in-cellRT-PCR)
细胞内RT-PCR
补充资料:Fanconi全血细胞减少综合征


Fanconi全血细胞减少综合征


  为常染色体隐性遗传。表现:矮小占56%,头小占43%,拇指发育不全到缺如占78%。皮肤不均匀色素沉着,并有随年龄增长而增加的倾向,尤其在肛门生殖器,腹股沟,腋窝及躯干等。小阴茎、小睾丸和/或隐睾,占男性病人的44%。全血细胞减少是此病常见特征。发病时间可见于婴儿至二十几岁。骨髓广泛发育低下,其中可见造血灶,一些红细胞呈巨红细胞性。白细胞减少主要为粒细胞减少。尸解经常见到脾发育不全。染色体断裂多。常有感染发生。早期治疗可以延长生命。
  
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