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1)  agrobacterium tumefaciens
根癌农杆菌
1.
The construction of the chsC-chitin synthase gene mutant of Aspergillus fumigatus by Agrobacterium tumefaciens-mediated transformation;
根癌农杆菌介导的烟曲霉几丁质合成酶chsC基因缺失突变体的构建
2.
Optimization of factors affecting genetic transformation of aspergillus fumigatus via Agrobacterium tumefaciens;
根癌农杆菌介导的烟曲霉转化条件的优化
3.
Agrobacterium tumefaciens mediated transgenic tetraploid Isatis indigotica carrying anti-insect gene from Pinella Ternata Agglutinin;
四倍体菘蓝转抗虫基因研究Ⅰ.根癌农杆菌介导的转基因菘蓝
2)  Agrobacterium
根癌农杆菌
1.
Agrobacterium-mediated Genetic Transformation System of Potato
根癌农杆菌介导的马铃薯遗传转化体系
2.
Effect of different culture temperatures on the growth of Agrobacterium and regeneration of rice immature embryos
不同温度培养对根癌农杆菌生长及水稻幼胚再生的影响
3.
barbadense shoot apexes mediated by Agrobacterium LBA4404 and EHA105 were studied.
采用根癌农杆菌LBA4404和EHA105介导,对海岛棉的茎尖再生体系及其转化影响因子进行了研究。
3)  Agrobacterium tumefacien
根癌农杆菌
1.
Studies on the Factors Affecting Agrobacterium tumefacien Mediated Transformation in Stem Segments of Potato;
根癌农杆菌介导的马铃薯茎段遗传转化条件的研究
2.
High transformation frequency was obtained when the leaves were pre-cultured for 2 d in the dark,then inoculated with Agrobacterium tumefaciens at A600 about 0.
采用根癌农杆菌介导的叶圆片遗传转化方法,建立了盆栽小菊稳定而高效的遗传转化体系。
3.
For the first time,the aiiA gene was transferred into Dendrobium by Agrobacterium tumefacien EHA105.
从Bt菌中克隆出aiiA基因,并装载入植物表达载体pCAMBIA1301,构建出可用于转入石斛兰的pSAN载体;首次利用根癌农杆菌EHA105将pSAN载体转入石斛兰,并获得潮霉素抗性苗。
4)  A. tumefaciens
根癌农杆菌
1.
GENETIC TRANSFORMATION OF Gypsophila paniculata BY A. tumefaciens;
根癌农杆菌在丝石竹上进行基因转化
2.
After 2 d of cocultivation with A.
本研究建立了油菜农杆菌转基因技术体系,用根癌农杆菌(Agrobacteriumtumefaciens)共培养法将PEP反义基因导入甘蓝型油菜(BrasicanapusL。
3.
The transformation of the seed-germinating embryoes of HeiNong 36 soybean was conducted with the A.
将含有35s启动子-Gus基因-ipt基因-Nos基因的根癌农杆菌LBA4404,做为供体。
5)  Agrobacterium tumefactions
根癌农杆菌
1.
Establishment of an Efficient and Stable Cabbage Transgenic System Mediated with Agrobacterium tumefactions and the Transformation of the CpTI gene;
根癌农杆菌介导的甘蓝高效稳定的遗传转化系统的建立及对CpTI基因转化的研究
2.
The anti-ACC gene was ransformed into Zixyphus jujuba by a efficient Agrobacterium tumefactions mediated system developed in this study after studying the transformation factors influencing.
经比较影响根癌农杆菌介导ACC合成酶反义基因转化枣树的各种因素后 ,建立了稳定的转基因实验体系。
3.
The gene for CaM and CaMBp was transferred into tobacco leaf disks with help of Tiplasmid of Agrobacterium tumefactions.
利用含有四环素阻遏蛋白,具有卡娜霉素抗性的烟草种子为实验材料,采用根癌农杆菌介导转化中的叶圆盘法,将外源基因导入烟草愈伤组织,建成可诱导表达细胞质和细胞核CaM/CaM结合多肽的转基因烟草体系。
6)  Agrobacterium-mediated
根癌农杆菌介导
1.
Agrobacterium-mediated transformation of pepper B162 with coat protein gene of cucumber mosaic virus(CMV-CP) was studied.
以辣椒品种‘B162’为试材,探讨了通过根癌农杆菌介导法将黄瓜花叶病毒外壳蛋白(CMV-CP)基因转化辣椒的适宜条件。
补充资料:农杆菌属
分子式:
CAS号:

性质:又称农杆菌属。根瘤菌科内的一属革兰氏阴性、能运动、有荚膜的好氧杆菌。细胞大小为(0.6~1.0μm)×(1.5×3.0μm)。生长温度25~28℃。在含糖培养基上产生黏性菌落。可广泛利用单糖、双糖和有机酸类。利用葡萄糖产酸而不产气。不能固氮。主要分布于根际土壤中。大多数菌种可感染双子叶植物的根并促使根或茎的增生,形成根癌等病症。G+Cmol%值为57~63。模式种为根癌土壤杆菌(A. tumefeciens),它的致癌作用是由孔质粒引起。目前Ti质粒已成为植物遗传工程研究中的重要载体,用以传递优良的外源遗传性状以培育植物良种。

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