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1)  Gene sequencing
基因测序
1.
Results LOH in Fas gene exon 8 were identified in all patients with keloids by PCR-SSCP, Gene sequencing proved these mutations.
结果 所检测的 15个瘢痕疙瘩标本 10 0 %有Fas基因外显子 8的杂合丢失 ,基因测序发现 11个标本共 4个位点存在基因突变。
2.
Gene sequencing and homology analysis show that these novel enzymes share remarkable homology with the amylase family.
基因测序及同源性分析表明这些新酶与淀粉酶家族具有很强的同源性。
2)  DNA sequencing
基因测序
1.
Objective: To establish a simple and reliable method to detect HBV genotype and HBV Lamivudine-resistant mutance,which is based on HBV S gene and YMDD segment in HBV P gene by DNA sequencing.
目的:利用基因测序技术,根据HBV S基因序列和HBV P基因区YMDD基因序列的特征,建立一种既可以诊断HBV基因型,又能检测拉米呋啶耐药突变的简便、可靠的方法。
2.
The positive clones were screened by agarose electrophoresis,and the inserted sequence in the recombinants was detected by DNA sequencing.
方法:以mU 6pro为载体,化学合成shRNA表达模板,并定向克隆到载体的U 6启动子下游,琼脂糖凝胶电泳法筛选阳性克隆,基因测序法检测插入模板序列的正确性。
3.
Gene mutation were analysised by Single Strand Conformation Polymorphism(SSCP) and DNA sequencing.
基因测序检测到PAX9基因外显子4中2个单核苷酸多态性位点:rs12881240、rs4904210;MSX1基因的2个外显子和PAX9基因其余3个外显子均未发现变异。
3)  Sequencing [英]['si:kwənsiŋ]  [美]['sikwənsɪŋ]
基因测序
4)  Genome sequencing
基因组测序
1.
A highlight of rice genome sequencing and its i nf ormation were presented.
综述了水稻基因组测序的工作进展和水稻基因组信息。
2.
This paper reviews recent relevant bifidobacterium plasmid isolation, vector, genome sequencing and gene therapy in the application of research progress.
综述了近年来有关双歧杆菌质粒分离、载体构建、基因组测序以及在基因治疗上的应用等研究进展。
5)  Sequencing of 18S rRNA gene
18SrRNA基因测序
6)  complete genome sequencing
全基因测序
补充资料:DNA测序
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性质:确定一段DNA中碱基排列的次序。一般用双脱氧测序法,另外还有一种马克西姆-吉尔伯特法,是依据四个碱基对不同试剂,如哌啶及肼的不同的反应能力实现的。可利用克隆载体上接头的两侧引入已知序列(例如Sp6,T7,T3的启动子序列),当插入目的片段后,即可利用此已知序列的寡核苷酸作引物。常用于未知序列DNA片段的测序。

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