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1)  Oncogene amplification
癌基因扩增
2)  gene amplification
基因扩增
1.
Use of Gradient PCR in Gene Amplification Study;
梯度PCR在基因扩增研究中的应用
2.
Research on gene amplification of BMP-2 in jawbone tumors by RT-PCR;
颌骨肿瘤中骨形成蛋白-2基因扩增的RT-PCR研究
3.
The investigation of STK15 gene amplification and overexpression in laryngeal squamous cell carcinoma;
喉癌中STK15基因扩增及表达的研究
3)  PCR
基因扩增
1.
The microbial community structure and microbial safety in three water sources including lightly polluted XL reservoir,moderately polluted LZ lake and seriously polluted XZ river,were investigated with PCR/DGGE molecular biology method.
利用基因扩增—梯度变性凝胶电泳(PCR—DGGE)分子生物学方法对污染程度较轻的XL水库、污染程度中等的LZ湖和污染严重的XZ河等三类水源的微生物群落结构以及微生物安全性进行了调查研究。
2.
The microbial community structures in polluted MK river,XL reservoir and BJ water plant were studied with polymerase chain reaction/denaturing gradient gel electrophoresis(PCR/DGGE) molecular biology method.
应用基因扩增—梯度变性凝胶电泳(PCR—DGGE)分子生物学方法对深圳市XL水库水源和BJ水厂处理工艺中的微生物群落结构特征进行了比较研究。
3.
Allelic data for the D17S30 Locus was obtained by using PCR and Subsequent analysis with high resolution, horizontal PAGE technic and silver staining,In a sample of 130 unrelated Chinese Han, heterozygosity of the D17S30 Locus was 82.
采用基因扩增结合电泳银染技术分析130名汉族群体DNAD17S30遗传多态性,发现了13个等位基因,基因频率为0。
4)  genome amplification
基因组扩增
1.
The genome of sindbis virus was amplified by random PCR and MDA strategies respectively, and the products were used as template to amplify specific gene in it to test the quality of the two genome amplification method; then the genome amplification products of sindbis virus were labeled with fluorescence dye and detected with microarray.
为了摸索均衡的病毒基因组扩增方法,建立高通量的病毒检测基因芯片技术平台,本研究以甲病毒属的辛德比斯病毒作为检测模型,分别以随机PCR扩增法和MDA(multipledisplacementamplification)扩增法扩增病毒基因组,并以两种扩增产物作为模板,扩增辛德比斯病毒的特异基因片段以初步验证基因组扩增的均衡性;然后将两种基因组扩增产物标记荧光染料后与基因芯片进行杂交;结果表明从两种基因组扩增产物中正确扩增出了辛德比斯的特定基因片段,作为探针可与基因芯片上的靶标基因特异性结合;基因组扩增产物与基因芯片进行杂交,可成功检测到甲病毒属的特异性信号,充分说明随机PCR扩增法和MDA扩增法扩增病毒基因组可用于病毒性病原体的基因芯片检测。
5)  Gains of chromosome
染色体基因扩增
6)  Whole genome amplification
全基因组扩增
1.
Preimplantation genetic diagnosis for β-thalassemia using whole genome amplification;
应用全基因组扩增技术对β-地中海贫血进行胚胎植入前遗传学诊断
2.
Methods Whole genome amplification (WGA) was performed for trace DNA using modified IPEP, and the amplified products were quantitated by real-time quantitative PCR as well as genotyped by AmpFLSTR~ Indentifiler~ kit.
方法用改良IPEP法对痕量样品DNA进行全基因组扩增(WGA),扩增产物用实时荧光定量PCR技术定量、用AmpFLSTR~ Indentifiler~试剂盒作基因型检测。
3.
Objective To establish a method for whole genome amplification (WGA) based on (multiple displacement amplification MDA), and achieve DNA analysis of the human genome from low copy number (LCN).
目的建立基于多重置换扩增(MDA)技术的全基因组扩增(WGA)方法,实现对低拷贝数(LCN)DNA样品进行分析。
补充资料:基因扩增
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性质: 又称基因放大。是指在一定条件下,把特定的基因(群)加以大量复制的现象。(1)高等生物为了表达在特定发育期中的某一特定性状,决定该种性状的基因数便会大幅度增加。如非洲爪蟾的卵母细胞中的rRNA基因,比体细胞的数量约大1500倍。(2)在动物的培养细胞中,对双氢叶酸还原酶(DHFR)的抑制剂甲氨蝶呤具有耐药性的细胞,已知其DHFR基因增加达1000个拷贝,已使用于对基因扩增机制的分析等。(3)在细菌中,已知有质粒数(拷贝数)增加的现象。基因扩增的这一形式,可被用来增加目的基因的产量等(基因剂量效应)。

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