Influence of different parameters of electrical fusion and activation on development of porcine somatic cell nuclear transfer embryo;

Firstly,reviewed the domestic and foreign progress of transgenic cattle produced by pronuclear microinjection and somatic cell nuclear transfer,Secondly,introduced the application of the new technologies,including gene targeting,RNAi and MMCT et al,in production of transgenic cattle and safe appraisal of transgenic cattle and its products.

To examine the effects of somatic cell serial nuclear transfer (SCSNT) on buffalo somatic cell nuclear transfer (SCNT),SCSNT was conducted with the buffalo fetal cells as the donor cells.

Effects of ultraviolet irradiation on in vitro development of parthenogeneticand somatic nuclear transferred embryos in bovine;

Epigenetic modification related to somatic nuclear transfer

So the current research was designed to study main items of pig somatic nuclear transfer systematically such as somatic donors, recipient oocyte, culture of cloned embryos and embryo transfer.

The oocytes having specific genetic background recovered by ovum pick up(OPU) technology, were used as recipient cells of somatic cell nuclear transfer(SCNT) to receive donor nuclei from different origins.

The purpose of the study was to investigate in vitro development,quality and frozen tolerance of bovine somatic cell nuclear transfer(SCNT) embryos in two defined culture media(IVD101 and G1/G2).

Later, pEBB was trasfected into bovine fetal fibroblasts and positive cells was selected to produce transgenetic cloned embryos by Somatic Cell NuclearTransfer(SCNT).

In comparison with the traditional method, this technique of somaticcell nuclear transfer has many advantages.

In order to determine the appropriate electric fusion parameters for porcine somatic cell nuclear transfer, four experiments were conducted in the present study, in which in vitro matured porcine oocytes were used as recipients and porcine granulosa cells were used as donors.