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1)  PCR-flow through reverse dot blot
PCR-流过式反向斑点杂交
2)  PCR-Reverse dot blot hybridization
PCR-反向点杂交
3)  PCR dot-blot hybridization
PCR-斑点杂交
1.
In this study, copepoda and its resting eggs in shrimp culture ponds were detected using PCR dot-blot hybridization and nest-PCR for the presence of WSSV.
本研究利用PCR-斑点杂交和巢式PCR法检测野外发病对虾养殖池塘中桡足类及其休眠卵,以及由这些休眠卵孵化出的幼体携带病毒情况,发现桡足类由卵到成体各阶段中均有阳性个体,而且池塘中在春天放养对虾苗前桡足类就出现阳性个体。
4)  reverse dot blot hybridization
反向斑点杂交
1.
Rapid detection of mycobacterium tuberculosis isoniazid-resistant genes by membrane reverse dot blot hybridization;
应用膜反向斑点杂交技术检测耐异烟肼结核分支杆菌基因型
5)  Reverse dot blot
反向斑点杂交
1.
Methods: By the way of PCR combined with reverse dot blot,carcinomas themselves(group A),the epidermal cells(group B) on the carcinomas and the vein bloods(group C) of 19 cases with oral squamous cell carcinoma were tested the HPV types.
方法:应用PCR-反向斑点杂交法检测19例口腔鳞癌患者病变组织HPV阳性的瘤体表面上皮细胞、血标本的DNA,对3组的阳性率进行比较。
2.
Methods Sixty-three cases highly suspec-tive of thalassemia were determined with the methods of ploymerase chain reaction(PCR) and reverse dot blot(RDB) for the type of gene mutation.
方法对63例高度疑诊为地贫的患儿采用多聚酶链反应(PCR)、反向斑点杂交法(RDB)进行基因检测。
3.
Then,PCR-Reverse Dot Blot was used to detect the types of human papillomavirus.
方法:运用PCR-反向斑点杂交法,检测桂林地区15例口腔黏膜白斑患者和21例正常口腔黏膜标本的DNA,对两组的阳性率进行χ2检验。
6)  reverse dot blot hybridization
膜反向斑点杂交
1.
Detection of mycobacterium tuberculosis ethambutol-resistant genes by membrane-reverse dot blot hybridization;
膜反向斑点杂交技术检测结核分枝杆菌耐乙胺丁醇基因型
补充资料:反向PCR
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性质:一种从基因组DNA中某一已知序列两侧扩增其侧翼未知DNA的序列的方法。用适当的限制酶切开基因组DNA,得到某一含有已知序列的片段,将其自身连接环化,使该已知DNA两端的DNA序列连在一起,应用与已知DNA序列两末端同源的寡核苷酸为引物,可使未知DNA得到扩增。从基因组DNA的角度看它的扩增方向与普通的PCR相反,故名。该法已有人用于染色体步读法(chromosome walking)。

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