Objective To establish an immune-blotting method of detecting anti-keratin antibody(AKA)and discuss its applicability and practicability in clinic.

An analysis and study of immunoblotting of different parts of protein of Fasciola hepatica;

An analysis of 2-D electrophoresis and immunoblotting of 18.6kD protein antigen of Fasciola hepatica;

Preparation of anti-human PD-1 antiserum with high-affinity and its application in PD-1 immunoblotting analysis;

Comparison on ELISA,Electroselenium method and Western blot for detection of anti-HIV antibody;

Estimation of postmortem interval by determination of troponin I using Western blot technique in human pectoralis major;

Diagnosis of Bovine Spongiform Encephalopathy and Scrapie by Western blot;

Liver stage proteins were analyzed by Western-blot.

Pure fimbriae were verified by Western-blot with monoclonal antibody (Mab) of F107.

pylori with coccoid variety by means of prolonged incubation and subinhibitory antibiotics, and then, studied the differences between the variety strain and its prototype through SDS-PAGE, Western-blot, furthermore, analysing the diferences of four virulent genes by PCR and PCR-SSCP respectively.

Development of Immunoblot Kit for the Detection of Anti,Toxoplasma Antibodies;

Analysis of immunoblot patterns of IgM and IgG antibodies in human sera of patients infected by Toxoplasma gondii;

Experimental research on protein immunoblot assay;

The SDS-PAGE and Western Blotting techniques were used to detect the expression level of HSP70 in the fish gills.

Methods The RT-PCR for AQP1 mRNA, Western blotting for protein, and immunohistochemistry for distribution in the seminal vesicle and prostate gland on postnatal day 15, day 35 and day 70 were carried out.

The mRNA and protein expression of DDC were examined by reverse transcriptase polymerase chain reaction (RT-PCR) and Western blotting methods, respectively.