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1)  cotransfection
共转染
1.
Plasmids cotransfections to non-attaching-wall mammalian cells using calcium phosphate method;
磷酸钙法对哺乳动物非贴壁细胞的质粒共转染
2.
Comparison Study on Plasmid Transfectionand Plasmids Cotransfection to CulturedMammalian Cells;
质粒单独转染与共转染哺乳动物培养细胞的对比研究
3.
The output of AcNPV budded virus was increased 2~13 times by cotransfection of Plutella xylostella Granulosis Virus (PxGV) DNA and Autographa californica Nuclear Polyhedrosis virus (AcNPV) DNA in S.
小菜蛾颗粒体病毒(PlutelaxylostelaGranulosisVirus简称PxGV)DNA与苜蓿银蚊夜蛾核型多角体病毒(AutographacalifornicaNuclearPolyhedrosisVirus简称AcNPV)DNA共转染S。
2)  Co-transfection
共转染
1.
DNA co-transfection,nude mice tumorig enicity assay,Southern hybridization,polymerase chain reaction(PCR)and DNA sequence analysis were employed to study HENE-DNP,a human embryonic nasopharygneal epithelial cell line transformed by DNP.
采用DNA共转染、裸鼠致瘤性试验、Southern杂交、PCR测序和序列同源性比较分析等,对DNP转化的人胚鼻咽上皮细胞株HENE-DNP进行研究。
2.
With lipofectamine- mediated co-transfection technique, peNOS-Lu.
方法以基因重组技术构建人eNOS基因启动子区(-1~ -1 600 bp)驱动的萤火虫荧光素酶报告基因载体peNOS-Luc,采用脂质体介导的细胞基因共转染技术将peNOS-Luc、空载体pGL2-Basic和β-半乳糖苷酶表达质粒pCMV-β共转染NIH3T3细胞,用细菌脂多糖(LPS)、tPNS和转移生长因子β1(TGFβ1)等因子分别刺激转染后的NIH3T3细胞,检测并比较荧光素酶/β-半乳糖苷酶活性,以确定LPS、tPNS和TGFβ1对人eNOS基因启动子转录活性的影响。
3.
Method With human embryonic kidney (HEK) 293 cells as the target and the assis-tance of lipofectamine-mediated co-transfection techniques and luciferase reporter gene systems, FLAG-tagged p38 isoforms (namely FLAG-p38α, FLAG-p38β, FLAG-p38γand FLAG-p38δ) in pcDNA3, pcDNA3, piNOS-Luc and pCMV-βwere transfected into HEK 293 cells, and the relative activity of lucif.
方法以人胚胎肾293(HEK 293)细胞为靶细胞,采用脂质体(LPS)介导的细胞基因共转染技术、荧光素酶报告基因技术,分别将FLAG-tagged p38 MAPK 4种亚型、含有鼠iNOS基因启动子区的荧光素酶报告基因质粒(piNOS-Luc)、空载体(pcDNA3)、β-半乳糖苷酶表达质粒(pCMV-β)共转染,检测并比较荧光素酶相对活性。
3)  Co-transfect
共转染
1.
Recombinant Chinese hamster ovary (rCHO) cell line expressing high-level recombinant human bone morphogenetic protein 2(rhBMP-2) was constructed by co-transfecting the expression vectors pCDNA3.
1(+)-hBMP-2,与质粒pSV2-dhfr共转染CHO-dhfr-细胞,以含有700μg/mLG418的IMDM进行选择性培养,筛选抗性克隆,并用MTX扩增,提高rhBMP-2的表达量。
4)  gene cotransfection
基因共转染
5)  The fat altogether extension dyes
脂质体共转染
6)  induction cell-based transfection assay
体外共转染报告系统
补充资料:世间转法转智转无尽
【世间转法转智转无尽】
  世间转者,谓展转摄前众生界、世界、虚空界也。法转者,谓展转摄前法界、涅槃界、佛出现界也。智转者,谓展转摄前如来智界,心所缘佛智所入境界也。盖此三转,皆言无尽者,以世法智之三种,展转含摄,无有穷尽,是名世间转、法转、智转无尽。
说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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