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1)  laser capure microdissection(LCM)
激光显微捕获(LCM)
2)  Laser capture microdissection (LCM)
激光捕获显微切割技术(LCM)
3)  the lcm
激光显微捕获
4)  laser capture microdissection
激光捕获显微切割
1.
Application of laser capture microdissection to capture pure cells in lung cancer and their paired normal lung tissues;
应用激光捕获显微切割技术制备肺癌蛋白质组学研究样品
2.
Proteomic analysis of esophageal squamous cell carcinoma by laser capture microdissection;
运用激光捕获显微切割对食管鳞状上皮癌蛋白质组的分析
3.
Proteomic analysis of hepatocellular carcinoma and cholangiocarcinoma by laser capture microdissection;
运用激光捕获显微切割对肝细胞癌与肝内胆管癌蛋白质组的分析
5)  laser capture microdissection
激光捕获显微切割技术
1.
To screen metastasis-associated biomarkers of lung AdC, laser capture microdissection (LCM) was used to purify the cancer cells from primary lung AdC with (LNM AdC) and without metastasis (non-LNM AdC) according to clinical diagnosis of lymph node metastasis and distant metastasis.
为了筛选潜在的肺腺癌转移相关分子标志物,依据临床诊断选取无转移的肺腺癌组织和有转移的肺腺癌组织作为研究对象,首先采用激光捕获显微切割技术(laser capture microdissection,LCM)对两组肺腺癌组织中的癌细胞进行纯化,再利用荧光差异凝胶电泳技术(two-dimensional differential in-gel electrophoresis,2D-DIGE)分离无转移肺腺癌组和有转移肺腺癌组的癌细胞总蛋白,通过Decyder软件分析两组差异表达的蛋白质点,质谱(mass spectrometry,MS)对差异表达的蛋白质点进行鉴定,Western blot验证部分差异蛋白annexin A1,annexin A2,annexin A3,B23和S100A9的表达。
2.
Objective To investigate the feasibility of laser capture microdissection(LCM) to offer homogeneous transitioncal cell and extracellular matrix(ECM) for the proteomic research of superficial bladder cancer and to determine the relationship between the shoots and the total protein.
目的探讨使用激光捕获显微切割技术(LCM)提供的均一性的肿瘤、正常移行上皮细胞以及细胞外基质(Extracellular ma-trix,ECM)对浅表膀胱移行细胞癌进行蛋白质组学比较研究的可行性。
3.
Screening for differentially expressed proteins in nasopharyngeal carcinoma by laser capture microdissection and proteomic analysis;
方法:采用激光捕获显微切割技术(laser capture microdissection,LCM)分别从NPC组织和正常鼻咽上皮组织(normal nasopharyngeal epithelial tissue,NNET)中纯化NPC细胞和正常鼻咽上皮细胞(normal nasopharyngeal epithelial cells,NNEC),应用二维凝胶电泳(2-DE)分离LCM纯化细胞的蛋白质,图像分析识别差异表达的蛋白质点,基质辅助激光解吸电离飞行时间质谱(MALDI-TOF-MS)和电喷雾电离串联质谱(ESI-Q-TOF-MS)鉴定差异蛋白质点,Western印迹和组织芯片(tissue microarray,TMA)免疫组织化学验证差异蛋白鳞状细胞癌抗原1(SCCA1)在两种组织中的表达水平。
6)  laser capture microdissection (LCM)
激光捕获显微切割
1.
Laser capture microdissection (LCM) is a novel technique that can be applied to obtain pure targeted cell subgroup or even a single cell quickly and precisely under the microscope, thus the problem of tissue heterogeneity in molecular analysis can be tackled successfully.
近年发展起来的激光捕获显微切割(lasercapturemicrodissection,LCM)技术可以在显微镜直视下快速、准确地获取所需的单一细胞亚群,甚至单个细胞,从而成功解决了组织中细胞异质性问题。
补充资料:激光显微光谱分析装置
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性质: 以激光为激发光源的光谱仪器,由激光光源和光谱仪两大部分组成。前者包括激光器、显微镜、辅助电极和电源柜。激光器产生激光,显微镜观察试样并聚焦激光束,辅助电极使激光气化的试样蒸气激发发光,电源柜为激光器和辅助电极的供电线路。光谱仪摄谱并记录产生的光谱。本装置可对试样的极微细部分进行元素的定性和半定量分析。

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