To investigate the pathogenesis mechanisms of Streptococcus suis type 2 (SS2) toxicity related factors and develop highly effective diagnostic methods, the dominant antigenic region of extracellular factor protein (EF) gene fragment was cloned into prokaryotic expression plasmid.

In order to establish an immunological method of detecting the extracellular factor (EF)from Streptococcus suis type 2 (SS2),the epf gene of SS2 of human origin strain ZYH24 isolated in Sichuan province was truncated and cloned to prokaryotic expression vector pGEX4T-2 and the expressed fusion protein rEF-GST with molecular mass (Mr) of 62 000 was expressed in E.

The gene fragment of 1585 to 2572 bp in length encoding the extracellular factor proteins was amplified from genomic DNA of Streptococcus suis type 2 strain SS 2 H isolated in Jiangsu province by PCR.

The pathogenicity of Streptococcus suis type 2 is associated with its virulent factors, including extracellular factor protein(EF).

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