In the study,the effects of different osmotic pressures and sorbitol concentrations in embryo culture medium on subsequent embryonic development of porcine oocytes in vitro fertilization were investigated and discussed.

The purpose of this study is to establish optimal, effective system for in vitro fertilization and embryo culture and improve the quality of embryo.

Methods: A retrospective analysis was performed on blastocyst culture and frozen-thawed blastocyst transfer cycles from October 2002 to December 2003 in Xiangya Hospital.

Studies on embryo culture of interspecific hybridization from Cymbidium lawianum and C.hookerianum;

Recovery of cucumber (Cucumis sativus L.) haploid plants through pollination by irradiated pollens and embryo culture;

Effects of different inoculation dates on ovule culture of three seedless cultivars;

Ovule culture and adventitious bud regeneration from immature cotyledons of apricot;

Medium has little effects on ovule culture.