Methods Rats were randomly divided into 7 groups: control group, peanut oil High fat Diet (PE-HFD), Lard High fat diet (LA-HFD), PE-HFD+4% Nattokinase extraction (NKE), PE-HFD+8% NKE, LA-HFD+4% NKE, LA-HFD+8% NKE.

Production of nattokinase and soybean isoflavone by natto strain;

Optimization of fermentation conditions for high-production nattokinase(NK);

Molecular Cloning and expression of natto kinase gene in Escherichia coli;

The optimal condition for natto kinase (NK)-fermenting was determined as follow: soybean as nitrogen source, fecula as carbon source, carbon-nitrogen ratio 3:1, 37℃, and pH6.

Nattokinase(NK) has strong fibrinolytic activity,its acute toxicity was tested in this paper.

In this study, we seperated 25 strains with nattokinase(NK) activity from the soybean fermentation diets with the protein-fibre plates method and 8 strains were selected for further detection.

Purpose Molecular cloning and expression of pro nattokinase gene with E.

Molecular clonging and expression of pro-nattokinase gene and nattokinase gene;