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1)  T7 promoter
T7启动子
1.
Construction of expression system controlled by T7 promoter at different expression level in mammalian cells;
在哺乳类动物细胞中呈现不同表达水平的T7启动子表达系统构建
2.
Methods cDNA of TIMP-1 was obtained by RT-PCR,and coloned PGEM-T vector,and identified by PCR and sequencing;and coloned recombinant plasmid PGEM-T/TIMP-1 was used as template to obtain cDNA of TIMP-1 with T7 promoter by PCR;and dsRNA of TIMP-1 was obtained by transcription.
方法 RT PCR获得TIMP 1cDNA ,克隆于PGEM- T载体上,PCR鉴定并进行序列分析;以PGEM- T/TIMP- 1重组质粒为模板,PCR获得带有T7启动子的TIMP 1cDNA ;体外转录获得TIMP- 1dsRNA。
3.
Flask_shaking experiments and batch fermentations were performed to investigate the effects of medium, inducer and glucose_feeding mode on bFGF formation directed by a T7 promoter.
在前期试验的基础上 ,重点探索培养基、诱导剂及葡萄糖添加方式对T7启动子指导下的bFGF合成的影响 。
2)  T7 promoter system
T7启动子系统
3)  T7 terminator
T7终止子
4)  promoter/non-promoter
启动子/非启动子
5)  promoter [英][prə'məʊtə(r)]  [美][prə'motɚ]
启动子
1.
A DNA Autonomous Replication Origin with Promoter Function in Yeast;
一个具有启动子功能的酵母DNA自主复制起始区
2.
Construction of recombinant of luciferase promoter reporter gene of endothelial-overexpressed lipopolysaccharide-associated factor 1;
人内皮细胞高表达脂多糖相关因子1启动子荧光素酶报告基因重组体的构建
3.
Effect of Aspirin on humanα_2(Ⅰ) collagen promoter induced by rhCD40L;
rhCD40L及阿司匹林对人I型胶原α_2(I)链启动子活性表达的影响
6)  35s promoter
35s启动子
1.
The quantitative competitive polymerase chain reaction (QC-PCR) system of using 35S promoter heterologous template for the detection of genetically modified maize was developed in this study.
建立了玉米转基因成分中35S启动子非同源模板的竞争定量PCR检测系统。
2.
However, the most generally used consititutive CaMV 35S promoter often fail in some incidences, especially for those genes in male organgenesis and male gametogenesis.
然而,在拟南芥遗传学研究中最常用的花椰菜花叶病毒35S启动子在花器官中,尤其在雄蕊中往往并不能做到持续而强烈地表达目的基因,所以有利用35S启动子无法互补突变体表型,或者观察不到异位表型的事例的报导。
3.
And also it is strong recommended that the biosafe construct should have no any uncertain element and exogenous DNA sequence such as antibiotics gene,35S promoter etc.
本研究以pCAMBIA1300载体为基础,基因操作去除pCAMBIA1300质粒上的潮霉素抗性基因和花椰菜花叶病毒的35S启动子序列,构建了两种只含有水稻蜡质基因启动子引导蜡质基因反义片段的表达载体,p13AWY-1和p13AWY-2。
补充资料:启动子
分子式:
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性质:(一)在DNA分子上,可被RNA聚合酶识别并与之结合的一段序列,位于结构基因的上游,在那里使转录起动。强的启动子可转录出更多的nRNA。因而在重组工作菌中常使用强制的启动子,以期取得更多的表达量。(二)一种致癌因素,在癌变过程的第二阶段或多阶段机制中造成第二阶段(促进阶段);一种辅助致癌物。

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