Study on the Recombining Conditions Between A and B Chains of “Mini-C” Human Proinsulin Analog and Human Proinsulin;

By using the site-directed mutagenesis technique,encoding-box of A11 amino acid residue and A12 amino acid residue of human proinsulin gene are exchanged and the [CysA11Ser,SerA12Cys]-human proinsulin mutant gene is constructed.

A goatβ-lactoglobulin(BLG) regulatory sequences, enhancer sequence, mark gene (Neo gene) and human proinsulin genomic DNA were used to construct human proinsulin mammary gland-specific expression vector.

Construction and identification of the human proinsulin gene recombinant expressing mature insulin in exopancreas;

Package of recombinant retroviral vector carrying modified human proinsulin gene and identification of virus titer;

Objective To study primarily the safety of transfering recombinant human proinsulin gene plasmid(RHPGP)into diabetic rats induced by streptozotocin.

The new method is another way for the preparation of human proinsulin C-peptide.

Optimization of the renaturation conditions for recombinant human proinsulin inclusion bodies expressed in E.

The Significance of Serum Proinsulin and Proinsulin to Insulin Ratio in Hyperthyroid Patients for the Diagnosis of Early Islet β-cell Dysfunction;

Change of concentrations of true insulin,proinsulin in patients with coronary heart disease,and the probable relationship to serum lipid;

Effect of cholera toxoid-proinsulin conjugate and cholera toxin B sub unit admixtures on spontaneous autoimmune diabetes;

Construction of animal model of transgenic mice expressing human insulin;

Research on the purification and characterization of the recombinant human insulin;

Chemical synthesis,cloning and expression of human insulin A chain and B chain gene separately in E. coli;