1)  intein
蛋白质内含子
1.
Use of Ssp dnaB Mini-intein as a Fusion Partner for Preparation of Recombinant Human Brain Natriuretic Peptide;
Ssp dnaB蛋白质内含子介导的重组人脑钠素的制备
2.
Phosphorylation of Bcl10 in Immune Cell Receptor Signaling & Use of Ssp DnaB Mini-intein as a Fusion Partner for Preparation of Recombinant Human Brain Natriuretic Peptide;
免疫细胞受体信号转导中Bcl10的磷酸化作用& Ssp dnaB蛋白质内含子介导的重组人脑钠素的制备
2)  intein
蛋白内含肽
1.
In order to obtain the recombinant Tp-5 cyclic analogue [cyclo-(Cys-Arg-Lys-Asp-Val-Tyr-),cTP]efficiently by intein-mediated single column purification, a gene encoding 6-amino acids peptide was designed, synthesized and cloned into Escherichia coli expression vector pTWIN1.
为了实现蛋白内含肽(Intein)介导的重组环状胸腺五肽结构类似物[cyclo-(Cys-Arg-Lys-Asp-Val-Tyr-),cTP]的高效制备,设计并合成编码6个氨基酸的cTP基因,克隆到表达载体pTWIN1,重组表达质粒pTW-cTp转化E。
3)  intein
内含肽
1.
Research and application advancement of intein;
内含肽的研究及应用进展
2.
After the MAX-intein-CBD fusion protein was purified by chintin-affinity chromatography, the self-cleavage activity of the intein was induced by β-mercaptoethanol and the recombinan.
用诱导剂IPTG诱导由目的多肽、内含肽和几丁质结合域(Chitin binding domain,CBD)组成的"三元"融合蛋白表达;用几丁质珠亲和层析纯化了裂解液中的融合蛋白,用β-巯基乙醇切割融合蛋白,获得目的蛋白。
4)  intein
蛋白质内含肽
1.
In this review, the discovery, characteristics, identification, splicing mechanism of inteins and its biological significance were summarized.
蛋白质内含肽是存在于前体蛋白质中的一段多肽链,靠自我剪切的方式从前体蛋白中释放出来。
2.
Protein splicing, which is an intein mediated posttranslational processing, involves a series of intramolecular cleavage-ligation reactions.
蛋白质剪接是蛋白质内含肽介导的,一种在蛋白质水平上翻译后的加工过程,它由一系列分子内的剪切-连接反应组成。
3.
The progress in the mechanism of selfsplicing, structural characteristic, homing endonuclease and the application of intein were summarized.
蛋白质内含肽是能够自我剪接的一段多肽链。
5)  intein
intein
1.
When fused at intein s C-terminus,single-chain Fv(scFv) against human bladder carcinoma can be purified in a single chromatographic step because chitin binding domain(CBD) fused at intein s N-terminus can bind to the chitin resin and intein possess a inducible self-cleavage activity to separate scFv from the affinity tag.
将抗膀胱癌单链抗体 (PG)融合在蛋白内含子 (intein)的C末端 ,利用intein的N末端具有的chitinbindingdomain(CBD)对几丁质柱的吸附特性及intein的自切割活性对PG进行一次性纯化。
6)  intein
蛋白质剪切
1.
During the splicing process the internal protein fragment, intein, triggered the self-excision from the precursor protein and the concomitant ligation of the flanking protein fragments, exteins.
蛋白质剪切是一种翻译后修饰事件 ,它将插入前体蛋白的中间的蛋白质肽段 (Intein ,internalproteinfrag ment)剪切出来 ,并用正常肽键将两侧蛋白质多肽链 (Extein ,flankingproteinfragments)连接起来。
参考词条
补充资料：内含子
分子式：
CAS号：

性质：又称沉默DNA（silent DNA）。真核基因中的非翻译区，它不被表达于蛋白质分子或成熟的mRNA中。内含子把单个真核基因分成许多不连续的区域。内含子也可见于某些前核基因组，但较为少见，且也有许多调节功能。由核RNA转录产生的为不均一核RNA(hnRNA)含有内含子，经特殊的酶（如ribozyme）作用切去内含子序列，然后剩余的外显子（exon）被连接酶拼接成为成熟的mRNA。

说明：补充资料仅用于学习参考，请勿用于其它任何用途。