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1)  low pressure cation exchange chromatography in conjunction with gradient elution system
低压离子交换层析梯度洗脱分析系统
2)  gradient elution analysis
梯度洗脱分析
3)  ion-exchange chromatography
离子交换层析
1.
Formation of dimers in refolding of recombinant human interferon-γ by ion-exchange chromatography.;
离子交换层析复性重组人γ-干扰素折叠二聚体的形成
2.
Acetylcholinesterase(AChE) of Bombyx mori Linaeus,separated from the crude extract,was purified to electrophoretic homogeneity by Sephadex G-25 chromatography,DEAE-Sepharose Fast Flow ion-exchange chromatography and Sephacryl S-200 gel filtration,respectively.
采用了Sephadex G-25层析,DEAE-Sepharose Fast Flow离子交换层析和Sephacryl S-200凝胶过滤层析,对家蚕头部粗酶匀浆液中的乙酰胆碱酯酶进行纯化,得到的样品经聚丙烯酰胺凝胶电泳(PAGE)和SDS-PAGE检测均为一条带,用SDS-PAGE法测得其分子量为77。
3.
Then,BMP was isolated and purified by the methods of D301 ion-exchange chromatography(IEC)and Sephadex G-25 gel-filtration chromatography(GFC).
使用反相高效液相色谱(RP-HPLC)对重组酵母发酵液中一种牛肉风味肽(BMP)的稳定性进行了研究,并分别运用D301离子交换层析和Sephadex G-25凝胶过滤层析对其进行了分离纯化,最后利用RP-HPLC对目标肽进行分析检测。
4)  DEAE iron-exchanged chromatography
DEAE离子交换层析
5)  ion exchange chromatography
离子交换层析
1.
Study on the electrostatic interactions in cation exchange chromatography with molecular simulation;
基于分子模拟的离子交换层析中静电相互作用研究
2.
Crude inulinase preparation produced by Aspergillus ficuum SK004 was purified in a sequence of operations including decoloration by active carbon,ammonium sulfate fractionation,dialysis,ion exchange chromatography with DEAE—Sepharose CL—6B and column chromatography with Sephadex G—75.
通过活性炭脱色、硫酸铵分级沉淀、透析脱盐、DEAE-Sepharose CL-6B离子交换层析、Sephadex G-75凝胶色谱后,主要得到1种菊粉酶。
3.
To obtain high activity angiotensin converting enzyme inhibitor peptides (ACEIP), the method of ion exchange chromatography, gel filtration chromatography and RP-HPLC were used to separate the solution.
以玉米蛋白为原料酶解得到的酶解液具有降血压活性,为了得到高活性的降血压肽单体,本文采用了离子交换层析法、凝胶层析和RP-HPLC对酶解液进行了的分离纯化,得到了五个降血压多肽单体。
6)  anion-exchange chromatography
阴离子交换层析
1.
To obtain the main peanut allergen Ara h2, defatted process, centrifugation, anion-exchange chromatography and membrane dialysis were used during the purification with the crude ground peanut as material.
结果显示,采用阴离子交换层析方法,制取的Arah2蛋白纯度达90%,得率为21。
2.
Alcaligenes faecalis penicillin G acylase(AfPGA) was isolated from recombinant Escherichia coli DH5α,and purified by a combination of DEAE-Sepharose anion-exchange chromatography and(Butyl)-Sepharose CL 4B hydrophobic chromatography.
采用DEAE-Sepharose阴离子交换层析和Butyl-Sepharose CL 4B疏水层析,从重组大肠杆菌DH5α菌体中分离纯化得到粪产碱杆菌青霉素G酰化酶(AfPGA)。
补充资料:离子交换分析
分子式:
CAS号:

性质:利用离子交换反应进行分析的一种方法。对于离子交换分析,因离子交换反应是定量进行的,故用于定量分析。一般分析时,使试样溶液通过氢型阳离子交换树脂,将溶液中溶出的氢离子定量,求出试样溶液中的全部阴离子当量。当然要对溶液中起始存有的H+、OH-等进行修正。在这种情况下使用氯型阴离子交换树脂,可避免修正的困难。

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