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1)  Northern blotting
RNA印迹法
2)  Northern blot
RNA印迹
1.
Semi-quantitative RT-PCR and Northern blot analysis demonstrated that the expression of OsCOI1 in rice varied obviously after treatment with MeJA and ABA but was not affected by SA.
经半定量RT-PCR法和RNA印迹分析,表明水稻中OsCOI1表达水平在经茉莉酸甲酯和脱落酸处理后呈明显变化,但不受水杨酸和乙烯的影响,说明OsCOI1可能在茉莉酸信号途径和脱落酸途径中具有特定功能。
3)  Northern blot
RNA分子杂交印迹法
4)  RAN dot blot array
RNA斑点印迹阵列
1.
The RAN dot blot array for EGFR,c-myc and wtp53 were carried out.
分别对细胞硝酸纤维素膜(NCM)标本进行PCNA,VEGF的免疫斑点印迹阵列;对RNA硝酸纤维素膜标本EGFR,c-myc及wtp53的RNA斑点印迹阵列。
5)  Northwestern blot
RNA-蛋白质印迹
6)  Western blotting
Western印迹法
1.
Using western blotting and MTT chemosensi-tive testing, the relationship between the expression of NFkB family proteins, P53 and MDM2 in ovarian cancer cell lines and chemotherapy resistance of ovarian cell was ob.
培养13个卵巢癌细胞系,提取癌细胞蛋白,采用Western印迹法观察NFkB家族蛋白、P53和MDM2的表达,并用四氮唑盐法药敏试验观察与卵巢癌细胞化疗耐药性关系。
2.
Using the mouse antibody to Hsp70of human,pig,dog,etc,Hsp70was detected from seven randomly sele cted yeast strains by Western blotting.
随机抽取其中7株新生隐球菌,采用鼠抗人、猪、狗等的Hsp70抗体,通过Western印迹法来检测其Hsp70,根据实验信号强弱定性判断在不同刺激条件下的Hsp70产量。
3.
Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.
方法 :培养 13株卵巢癌细胞系 ,对其进行胞质、胞核蛋白质提取 ,Western印迹法观察NFκB家族蛋白及其抑制物的表达 ,并用MTT检测药敏、观察NFκB家族蛋白与卵巢癌细胞化疗耐药性关系。
补充资料:RNA印迹法
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性质:又称RNA印迹(法)。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜,尼龙膜等,并用探针进行杂交(称“北方”杂交)以显示目的区段的位置。显示RNA的大小及丰度。

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