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1)  Telomeric repeat amplification protocol
端粒重复序列扩增
1.
Telomerase activity was examined in 25 glioblastomas by telomeric repeat amplification protocol-silver staining.
[方法]采用端粒重复序列扩增 -银染法检查了25例胶质母细胞瘤的端粒酶活性。
2)  telomerase repeat amplification protocol (TRAP)
端粒重复序列扩增技术
1.
Methods: Telomerase repeat amplification protocol (TRAP) assay was used to examine telomerase activity in forty-eight brain gliomas and eight normal brain tissues.
方法 :应用端粒重复序列扩增技术 (TRAP)对 4 8例胶质瘤标本和 8例正常脑组织的端粒酶活性进行检测。
3)  PCR-TRAP
端粒酶重复序列扩增法
1.
Methods Telomerase activity was measured by PCR-TRAP in cancerous lesions brushing cells from patients with lung cancer and lesions brushing cells from patients with noncancerous pulmonary disorders;CEA in bronchial washing fluid and in blood was measured by radioimmunoassay ,in which the specimens were obtained by fiberoptic bronc.
方法 采用端粒酶重复序列扩增法和放免法测定分别检测肺癌病变组织 (12 0例 )、非癌性肺疾病 (30例 )纤支毛刷刷脱细胞的端粒酶活性和毛刷涮洗液、血CEA浓度 ,并和病理学及细胞学检查结果进行比较。
4)  TRAP [英][træp]  [美][træp]
端粒重复序列扩增法
1.
Methods The telomeric repeat amplification protocol (TRAP)and the silver staining assay were used to detect the telomerase activity in the tissue biopsy specimens and the brush biopsy specimens of bronchofiberscope from 35 patients with lung cancer and 35 patients with chronic bronchitis or benign pulmonary disease.
方法 采用以聚合酶链反应 (PCR)为基础的端粒重复序列扩增法 (TRAP) ,结合聚丙酰胺凝胶电泳银染法检测经纤维支气管镜取出的 3 5例肺癌患者肺癌组织和相应健侧肺支气管粘膜及 3 5例支气管 肺良性疾病患者支气管粘膜的端粒酶活性。
2.
Methods Telomerase activity was detected by a non-isotopic PCR-based TRAP (telomeric repeat amplication protocol) assay in tissue samples from 46 PGC,22 tumor-adjacent tissues and 19 benign lesions.
方法采用端粒重复序列扩增法(TRAP)检测了胆囊癌细胞系,46例原发性胆囊癌组织,22例癌旁组织,19例胆囊良性病变。
5)  telomeric repeat amplification protocol-enzyme linked immunosorbent assay
酶联免疫端粒重复序列扩增法
6)  Real-time fluorescent quantitative TRAP
实时荧光定量端粒重复序列扩增法
补充资料:简单重复DNA序列
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性质:又称简单重复DNA序列。是指DNA链内由2~4个核苷酸顺序串联重复排列的片段。广泛存在于真核生物基因中,可出现在基因编码区附近、非编码区或基因内。具有个体特征,是构成人基因组多态性的因素之一。其突变表现为重复顺序拷贝数的变化,而且有渐进累积的过程,突变频率与拷贝数有关。突变多数导致产生异常的蛋白质。已发现人类的五种遗传病与这种串联重复序列拷贝数变异有关:脆性X综合征、脊髓延髓肌萎缩症、强直性肌营养不良症、亨廷顿氏(Huntingtons)舞蹈病以及遗传性非息肉型结直肠癌等。

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