1) reverse transcription
反转录
1.
cDNA was synthesized by reverse transcription, and cDNA fragments larger than 400 bp were ligated with dephosphorylated arms of Agt11.
方法:从9901细胞中提取总RNA,分离mRNA,反转录合成双链cDNA,末端削平,和EcoR I适配子连接。
2.
In this study reverse transcription coupled with the polymerase chain reaction(RT PCR) has been established to detect hog cholera virus (HCV).
本研究建立了反转录聚合酶链反应 ( RT PCR)技术检测猪瘟病毒 ( Hog Cholera Virus,HCV)方法。
3.
A new strain of EHDV2 was studied by a series of experiments including extraction of RNA,reverse transcription, clone, PCR,purifiction of plasmid.
用美国新分离到的一株鹿流行性出血热血清Ⅱ型毒株，通过繁毒，提核酸，反转录，ＰＣＲ扩增，克隆，质粒增殖提纯，最后应用７脱氮ｄＧＴＰ试剂盒测定其ＶＰ７核酸基因序列。
2) RT-PCR
反转录PCR
1.
Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw.
方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。
2.
?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.
方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。
3.
Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained.
方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。
3) Reverse transcriptase
反转录酶
1.
OBJECTIVE:To investigate the diagnostic meaning of the human tolomerase reverse transcriptase (hTERT) gene expression level in peripheral blood mononuclear cells in non-Hodgkin’s lymphoma(NHL) patients.
目的 :探讨人外周血单个核细胞端粒酶反转录酶 (hTERT)基因的表达水平对于非霍奇金淋巴瘤 (NHL)诊断的意义。
2.
This study analyzed the characteristics of reverse transcriptase sequences of copia like retrotransposons in Amaranthus via PCR amplification and sequence approaches.
利用籽粒苋为材料 ,通过PCR扩增、克隆研究了copia类型反转录转座子的反转录酶序列在籽粒苋中的表现 ,结果表明 :(1)反转录转座子在苋属的 30个品系中同时检测到 ,说明反转录转座子在籽粒苋的不同种中普遍存在 ;(2 )对野生苋 (A 。
3.
In order to study the structural elements within the ε that is essential for initiating priming of HBV reverse transcriptase(P protein),all selected aptamers were subjected to in vitro priming assay and RNA secondary structure probing.
通过构建鸭乙肝病毒ε(Dε)的RNA文库并利用指数级富集的配体系统进化技术(SELEX)筛选的策略,获得与反转录酶(P蛋白)高度亲和的适配子(Aptamer),再通过体外引发实验和核酸酶切割方法测定全部适配子的RNA二级结构,以研究鸭乙型肝炎病毒(Duck Hepatitis B Virus,DHBV)中对启动P蛋白引发步骤至关重要的ε结构信息。
4) RT-PCR
反转录-PCR
1.
0kb DNA fragment was obtained by RT-PCR fr.
本研究以黑曲霉M-1为出发菌株，设计特异的引物，采用反转录-PCR技术直接从黑曲霉总RNA中反转录并扩增出约3。
5) RT PCR
反转录PCR
1.
cDNA fragment encoding human P34H was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector.
方法 :提取人附睾体部总RNA ,并以此为模板 ,进行反转录PCR获得编码P34H蛋白的基因片段。
2.
Methods:Tumor tissues and adjacent normal tissues in 41 cases of esophageal carcinoma were studied by using the reverse transcriptase polymerase chain reaction(RT PCR).
方法 :用反转录PCR(RT PCR)法检测 41例食管癌患者的癌组织、癌旁组织MMP 2mRNA的表达。
补充资料：反转录
分子式：
CAS号：
性质：又称逆转录。以RNA为模板产生DNA分子的过程。
CAS号：
性质：又称逆转录。以RNA为模板产生DNA分子的过程。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条