Prospects and advances in studies on hepatitis C virus (HCV) vaccine based on E2 glycoprotein;

To stably express hepatitis C virus (HCV) E2 glycoprotein in CHO cells and facilitate the detection and purification of the expression products, the gene fragment encoding N terminal 277 amino acids of this protein was fused to the fragment encoding hepatitis B virus (HBV) preS1(21—47) region and inserted into a secretion vector pSecTagB.

AIM:To induce granulocytic differentiation of murine leukemia-like 32D-P210 cells through heterogeneous nuclear ribonucleoprotei E2(hnRNP E2) decoy RNA aiming at the targeted blockage of the CCAAT/enhancer-binding protein αlpha(C/EBPα) gene abnormal translation, and to further investigate the potential molecular mechanisms.

Effect of over expression of HPV18 E2 protein on apoptosis and secretion of macrophages;

Cloning of genes transactivated by hepatitis C virus E2 protein by suppression subtractive hybridization technique;

Expression of the truncated E2 protein-encoding gene of classical swine fever virus in Escherichia coli and preparation of a monoclonal antibody against E2 protein;