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1)  eukaryotic plant expressing vector
真核植物表达载体
2)  eukaryotic expression vector
真核表达载体
1.
Construction and expression of eukaryotic expression vectors for mouse macrophage Ipr1 gene;
小鼠巨噬细胞Ipr1基因真核表达载体的构建及表达
2.
Cloning of rat FOXP3 gene and construction of its eukaryotic expression vector.;
大鼠FOXP3基因的克隆及真核表达载体的构建
3.
Construction of eukaryotic expression vector of gene of single chain variable fragment A7;
单链抗体A7基因真核表达载体的构建
3)  eukaryotic expression plasmid
真核表达载体
1.
Construction and ibentification of eukaryotic expression plasmid of shRNA on cMet;
肝细胞生长因子受体靶向shRNA真核表达载体的构建和鉴定
2.
【Objective】 To construct eukaryotic expression plasmid pIGM-CSF based on gene adjuvant hGM-CSF,and express in Hela cells.
【方法】采用聚合酶链反应从载体pORF-hGM-CSF中,扩增出hGM-CSF基因,克隆到双顺反子真核表达载体pIRES的多克隆位点B(MCSB)中,构建pIGM-CSF真核表达载体。
3.
Objective: To investigate the role of uPA in the development of hepatocellular carcinoma in vitro, we clone the coding sequence of human uPA gene, constract its eukaryotic expression plasmid,and transfect the human hepatocellular carcinoma cell lines.
目的:构建人尿激酶型纤溶酶原激活物(uPA)基因真核表达载体,并转染人肝癌细胞,为进一步体外研究uPA在肝癌发生发展中的作用奠定基础。
4)  shRNA eukaryotic expressing vector
shRNA真核表达载体
1.
Objective To investigate the effects of human melanin-concentrating hormone receptor 2(MCHR2) specific shRNA eukaryotic expressing vector on binding of radiolabeled ligand to MCHR2.
结论MCHR2基因shRNA真核表达载体能有效减少Bmax、升高Kd值,为利用RNA干扰技术研究人MCHR2基因功能奠定良好的实验基础。
5)  Eukaryotic expression vectors
真核表达载体
1.
Construction of Two Eukaryotic Expression Vectors of HBV X Gene with Different Selection Characteristics;
构建不同筛选特性的HBV X基因真核表达载体
2.
Construction of anti-mdr1 shRNA eukaryotic expression vectors.;
抗mdr1 RNA干扰真核表达载体的构建
3.
Objective:To construct ICAM-1 eukaryotic expression vectors.
目的:构建人ICAM-1-1真核表达载体pcDNA3。
6)  eukaryotic vector
真核表达载体
1.
Construction and identification of human PTEN and green fluorescent protein eukaryotic vector;
人PTEN绿色荧光蛋白真核表达载体的构建及鉴定
2.
Construction and identification of eukaryotic vector incorporated with wild-type PTEN;
野生型抑癌基因PTEN真核表达载体的构建及鉴定
3.
Construction of eukaryotic vector of the nucleocapsid protein gene of newcastle disease virus;
新城疫病毒NP蛋白的真核表达载体的构建
补充资料:植物克隆载体
分子式:
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性质:植物基因操作中之携带外来DNA的载体,并以可遗传的方式将它嵌入植物细胞之内。天然的载体有根癌根杆菌(Agrobacterium tumefaciens)的Ti质粒或发根农杆菌(A. rhizogenes)的Ri质粒以及DNA植物病毒。现在因Ti或Ri质粒太大,不易操作,常取其DNA复制及引发癌肿的基因与普通细菌质粒组成穿梭质粒,便于先在细菌(尤其是大肠杆菌)中操作、扩增,然后再引入植物。

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