Extra reference substance should not be participated in calculation of chromatographic fingerprint of traditional Chinese medicine;

4-Isopropylbenzoic acid was hydrogenated to give a mixture of cis-and trans-4-isopropyl-cyclohexanecarboxylic acid (76∶24),the mixture was transformed into the acylchoride with PCl5 ,and then condensed with D-phenylalaine,after recrystallization from acetonitrile,nateglinide cis-isomer was obtained which may be served as the reference in the quality control of nateglinide.

But “shark s fin cost one yuan” is not only the reference object of Xiang lin sister s tragic fate,but also a mirror responsing the nature of nation.

HPCE tech,which has been progressed very quickly in recent years,is one of the research method in CMM finger print,in this article,to sum up the application status of HPCE in finger print,compare differently from sample extract,electrophoresis condition,reference object,etc,provide reference for HPCE tech in CMM finger print research method.

Preparation of the reference material with low biosafety risk used for molecular detection of Cucumber green mottle mosaic virus