1)  DNA
体外扩增基因
2)  in vitro
体外
1.
Comparison of release characteristics between two mizolastine sustained release tablets in vitro;
2种咪唑斯汀缓释片的体外释放度比较
2.
Preparation of sinomenine gel and its skin penetration test in vitro;
青藤碱凝胶剂的制备及体外渗透性能研究
3.
Hysteroscopic findings and interventions in patients undergoing in vitro fertilization-embryo transfer;
宫腔镜技术在体外受精—胚胎移植失败患者中的应用
3)  in vivo
体外
1.
Study on the fibrinolytic speciality in vivo of extract from Dochi;
淡豆豉提取物的体外溶栓特性研究
2.
This paper studied the effects of lacto-bioactive peptides on the liver cells reproduction in vitro, the growth and non-special immune function of whole male in vivo through two feeding experiments.
本实验以全雄奥尼罗非鱼为试验对象,通过离体试验研究乳源活性肽对奥尼罗非鱼肝细胞体外增殖的作用,通过两期动物饲养试验研究乳源活性肽对罗非鱼生长和非特异性免疫功能的影响。
4)  external
体外
1.
The therapeutic effect of external high-frequency heat therapy on late cancer of digestive tracts;
体外高频热疗治疗晚期消化道肿瘤
2.
Based on the analysing deformation of external prestressed concrete beam, the total process from loading stage to fail stage was analyzed for external prestressed concrete beam by means of section force equation and deform accordance, and the ultimate stress of the tendon and the ultimate strength of the concrete beam were found out.
该文在分析体外预应力钢筋与混凝土主梁变形的基础上 ,通过截面内力平衡方程和变形协调关系 ,对体外预应力混凝土梁进行了从加载到破坏的全过程分析 ,得到了极限状态下体外筋的极限应力和混凝土梁的极限抗弯强度。
3.
In combating the serious corruptive cases with some Party officials, the Party should discipline the organization itself and its official members, and a performance appraisal system by external supervision can be introduced to reinforce and offset the on going Party member evaluating system so that the Party is governed with strict disciplines and supervision.
面对当前某些党员干部中存在严重的腐败现象 ,在坚持“党要管党”和“党管干部”的前提下 ,充分利用广大人民群众的监督作用 ,按照“三个”代表的要求 ,探索建立一种“体外”监督考评党员干部的制度 ,以加强和完善现行的党员民主评议制度 ,实现从严治党的目
5)  Vitro
体外
1.
Effects of Se on the Lipid Peroxidation of Liver and Brain in Vitro;
硒对肝脏脑体外脂质过氧化的影响
2.
Research of Hepatocyte-like Cells Formed from Human Umbilical Cord Blood Stem Cells in Vitro;
脐血干细胞体外分化为肝样细胞的实验研究
3.
To investigate the feasibility of induction mononuclear cells (MNCs) from human umbilical cord blood to hepatocyte-like cells with cytokines in vitro.
本实验探讨体外采用细胞因子诱导脐血单个核细胞(MNCs)定向分化为肝细胞的可能性。
6)  in vitro-in vivo extrapolation
体外-体外预测
参考词条
补充资料:基因体外扩增技术
分子式:
CAS号:

性质:又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性,分解为单链。在较低温度(37~63℃)与引物退火:然后变温到72℃左右,在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链,然后又变性→退火→延伸反复进行。引物大大过量,dNTP过量,酶在高温中是较稳定的,这样经过几十个循环,目标DNA片段就按2n数递增。用此法可从mRNA中,cDNA库中扩增出目标基因。过去一般合成500~1000bp较好,现已发展出大片段(75kb)的PCR,且差错甚少。PCR技术的发展还可用于定点突变,质粒重组及FLP等方面。这一技术的作用范围日益扩大,已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破,已在分子生物学、医学、法学等领域发挥了极大的作用。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。