1) prochymosin
[prəu'kaiməsin]
凝乳酶原
1.
Cloning and phylogenetic analysis of bovine prochymosin gene;
牛凝乳酶原基因克隆及序列的进化分析
2.
The Kpn I-Kpn I fragment of prochymosin gene was inserted into the phagemidvector:pBluescript.
以ｐｈａｇｅｍｉｄ，ｐＢｌｕｅｓｃｒｉｐｔ为载体，把牛凝乳酶原基因的ＫｐｎＩ－ＫｐｎＩ片段插入，将牛凝乳酶４５位Ｃｙｓ用Ａｌａ取代，根据遗传密码子的兼并性，在其附近引入限制性内切酶ＣｌａＩ位点。
3.
During the work of site\|directed mutagenesis at disulfide bond Cys206\|Cys210 of prochymosin,it was found that the corresponding template sequence had the potential to form a loop\|stem structure with free energy of -16 1 kcal/mol,which prevent the template from pairing with primer and,in turn,the synthesis of the mutated DNA strand.
在对凝乳酶原二硫键Cys2 0 6 Cys2 10进行定位突变过程中发现 ,在相应的模板序列中有自身形成自由能为 - 16 1kcal/mol的茎环结构倾向 ,妨碍与引物结合 ,从而难以合成突变的DNA ,采用快退火可解决此矛盾。
2) recombinant prochymosin
重组凝乳酶原
1.
By comparing PDI assisted renaturation with GSH/GSSG assisted renaturation of recombinant prochymosin, we found that: (1)Although the reaction rate of GSH/GSSG assisted renaturation was lower than that of PDI assisted renaturation, there was no difference between the final renaturation efficiencies of them.
对蛋白质二硫键异构酶与ＧＳＨ／ＧＳＳＧ促进重组凝乳酶原复性进行了比较。
3) preprochymosin
凝乳酶原前体
1.
Then based on blast the sequence of Ovis aries preprochymosin cDNA (Accession No: X53037) reported in GeneBank, two pairs of primers were designed to clone Xinong Saanen Goat preprochymosin with the 5 d postparturition abomasum cDNA as template by RT-PCR method.
本研究参照GenBank收录的绵羊凝乳酶原前体cDNA序列(GenBank No:X53037)设计RT-PCR、PCR与巢式引物,以新生5天的西农萨能羊公羔羊为研究对象,利用手术法采集羔羊皱胃组织,利用Trizol法提取皱胃组织总RNA,经过电泳鉴定后,以总RNA为模板利用RT-PCR方法获得西农萨能羊凝乳酶原前体cDNA序列,以cDNA序列为模板进行扩增和T载体连接测序。
4) chymotrypsinogen
[,kaiməu'trip'sinədʒən]
胰凝乳朊酶原
5) chymotrypsinogen
[,kaiməu'trip'sinədʒən]
胰凝乳蛋白酶原
6) rennet curdling
凝乳酶凝乳
补充资料：胰凝乳蛋白酶原
胰凝乳蛋白酶原 chymotrypsinogen
胰凝乳蛋白酶原 chymotrypsinogen 为胰凝乳蛋白酶的前体物质。牛的胰凝乳蛋白酶原a氨基酸残基245个，分子量24500。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条