The waxy gene is the key gene of the amylase synthesis.

We have previously reported that a 31 bp DNA fragment within the 5 upstream region of rice waxy gene could interact specifically with nuclear proteins extracted from immature rice seeds.

4 kb (-2118～+1291 bp) sequence located upstream of translationalstart site of rice waxy gene is sufficientto drive the expression of the GUS reporter gene in rice immature embryos.

Study on effect of detection of Wx gene in non-glutinous rice of Oryza sativa L. Indica by STS molecular marker;

After analyzing glutinous rice waxy gene sequence reported by previous studies, two pairs of dominant and codominant STS molecular markers based PCR were designed for distinguishing glutinous rice, according to the distinctions between glutinous rice wx gene and nonglutinous rice Wx gene.

Two chimeric plasmids with Wx gene promoter(from O.

An anti-Waxy gene was introduced into rice ‘Xiang Qing’ and transgenic plants were obtained.

Analysis on the characters of progenies of the rice transformed with anti-sense waxy gene;

【Method】 Anther culture pure lines containing antisense waxy gene were generated by Agrobacterium tumefaciens mediated co-transformation and followed by anther culture in transgenic rice variety.