Countering the unresolved problems of maize anther culture such as the low induction frequency, great differences among the genotypes, the factors which effected the maize anther culture were centered on and the culture system was established which was fit for culturing high induction frequency of main anther culture.

The result showed that the induction frequency of pollen callus was irrelevant to the volume of culture containers,and highly related to the density of pollens.

Studied Cuoho inducer 1 effect induced rate in different maize germplase,the resulted showed,the average haploid induction rate of different germplasm was 3.

10 mg/L NAA significantly improved the embryoid induced rate and ratio of mature embryos and NLN-13 liquid media+rational active carbon obviously promoted the embryoid formation and development.

5 minutes,which reduces the polluting rate and melting brown rate and improves the inducing rate of the bud.

Results indicated that the haploid inducing rate was significantly influenced by the silk length and pollination date.

From the researched result,many factors could influence the inducing rate of callus and the differentiations rate of the rebirth plant,such as:rice genotype,types and classes of culture medium、hormonal concentrations\'s ratio、illuminations、PH value and so on.