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1)  SYBR Green I real-time PCR
SYBR~ GreenI实时PCR
2)  SYBR Green I
SYBR GreenI
1.
Standard curve was established using a series dilution of quantified plasmids to measure EZH2 using SYBR Green I real-time fluorescent polymerase chain reaction and the characteristic of specific EZH2 amplicon was analysed by melti.
目的:建立SYBR GreenI实时荧光PCR定量检测人类EZH2基因的方法。
2.
According to the avian reticuloendotheliosis virus(REV) LTR sequence available in GenBank,a pair of specific primers were designed which target to the conserved region of LTR,and SYBR Green I-based real-time PCR was developed.
根据禽网状内皮组织增生症病毒(REV)长末端重复序列LTR基因保守区域设计并合成一对引物,建立基于SYBR GreenI模式的实时荧光PCR方法(Real-timePCR)。
3)  SYBR-GreenⅠ real-time PCR
SYBR-GreenⅠ实时荧光PCR
1.
The SYBR-GreenⅠ real-time PCR was established by the positive quality control.
利用阳性质控品建立SYBR-GreenⅠ实时荧光PCR方法,对反应体系进行优化,进行特异性和敏感性分析,并制作标准曲线。
4)  real-time fluorescent SYBR-Green PCR
SYBR-Green实时荧光PCR
5)  SYBR~ Green Ⅰ real-time PCR
SYBR Green Ⅰ实时PCR
1.
Bt genetically modified rice was identified with SYBR~ Green Ⅰ real-time PCR assays and three pairs of specific primers to establish a new detection technique for GMO.
为了建立转基因产品新型检测技术,采用SYBR Green Ⅰ实时PCR技术和三对特异引物,检测抗虫转基因水稻外源基因(CaMV35S,NOS,Cry1Ab/c)。
6)  multiplex SYBR Green-Ⅰ real time PCR
多重SYBR Green-Ⅰ实时荧光PCR
1.
Establishment of a multiplex SYBR Green-Ⅰ real time PCR assay for simultaneous detection of porcine reproductive and respiratory syndrome virus,porcine circovirus type 2 and porcine pseudorabies virus;
PRRSV和PCV-2以及PRV多重SYBR Green-Ⅰ实时荧光PCR检测方法的建立
补充资料:immune PCR
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性质: 通过应用一个对DNA和抗体具双重结合活性的接连分子使作为标志物的DNA分子特异地结合到抗原-抗体复合物上,从而形成一种特异性抗原-抗体-DNA复合物。附着的DNA标志物可用适当的引物进行PCR扩增。特异性PCR产物的存在证明DNA标志物分子特异性地附着于抗原-抗体复合物上,进而证明有抗原存在。目前最为敏感的检测方法,理论上可测得一个抗原分子的存在。

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