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1)  Phage antibody library technology
噬菌体抗体库技术
1.
Phage antibody library technology includes, amplify the full set variable region gene of antibody by PCR,connect the DNA fragments of antibody molecule with phage coat protein gene PⅢ or PⅧ,and then let the fusion protein be expressed on phage surface.
噬菌体抗体库技术是利用PCR扩增出抗体的全套可变区基因,将抗体分子DNA片断如Fab或单链抗体(ScFv)与噬菌体外壳蛋白基因PⅢ或PVⅢ连接,使融合蛋白表达于噬菌体颗粒的表面,经过“吸附-洗脱-扩增”过程富集筛选特异性抗体。
2)  Phage antibody display technology
噬菌体抗体展示技术
3)  Phage antibody library
噬菌体抗体库
1.
Construction of phage antibody library using sensitized in vitro B-lymphocytes of liver cancer patients;
用体外致敏联合EB病毒转化技术构建抗肝癌的人源Fab噬菌体抗体库
2.
Construction and preliminary identification of humanized Fab fragment phage antibody library against Schistosoma japonica;
人源性抗日本血吸虫噬菌体抗体库的构建及初步鉴定
3.
Construction of single-chain phage antibody library of cancer-bearing EL-4 mice;
EL-4荷瘤鼠单链噬菌体抗体库的构建
4)  T7 phage display library
T7噬菌体抗体库
1.
A T7 phage display library of human single-chain fragment of variable region (scFv) was constructed to screen antibodies against nucleocapsid protein (NP) of Hantaan virus.
结果表明,研究成功构建了人源抗NP蛋白T7噬菌体抗体库。
5)  phage display antibody library
噬菌体抗体库
1.
Construction and screening of phage display antibody library directed against the extracellular domain of human integrin β_3;
抗整合素β_3胞外区噬菌体抗体库的构建及筛选
2.
Construction of humanized phage display antibody library for human cytomegalovirus;
抗HCMV人源化噬菌体抗体库的构建
3.
Phage display antibody library technology, which has been widely used in the diagnosis and treatment of disease, and functional protein expression, was a high-performance, time saving, and effective technique for producing specific antibodies.
噬菌体抗体库技术是继单克隆抗体制备技术后具有换代地位的抗体制备技术,在功能蛋白的表达、疾病诊断和治疗等领域得到了广泛研究,用在农兽药等小分子抗体的制备上也获得了亲和力较高的抗体。
6)  Phage display library
噬菌体抗体库
1.
The functional binding antibodies to novel purinoceptor selected from phage display library;
从噬菌体抗体库中筛选血管内皮细胞新型嘌呤受体功能抗体的研究
2.
Objective To construct Fab phage display library against human B-lymphoma.
目的构建B细胞淋巴瘤Fab噬菌体抗体库。
3.
Objective The purpose of this study was to obtain the high-affinity single-chain Fv antibody against platelet membrane glycoprotein(GP) Ⅱb/Ⅲa from anti-activity platelet phage display library.
方法 从活化血小板免疫的小鼠脾淋巴细胞中提取总RNA ,反转录成cDNA后 ,用抗体可变区混合引物扩增全套轻、重链可变区基因 ,经重叠延伸反应 ,装配成单链抗体 (ScFv)基因 ,将其克隆到噬菌体载体pHEN1中 ,构建单链抗体噬菌体抗体库。
补充资料:免疫学技术乙型肝炎早期抗体的测定(见免疫学技术)




免疫学技术  乙型肝炎早期抗体的测定(见免疫学技术)
                   中国医学科学院病毒研究所提供
  [图]图

说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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