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1)  double layer culture
固液双层培养
1.
The effects of GA_(3)and IAA on the formation of stolons and produltion of microtubers,and the effects of solid culture,double layer culture and liquid culture on induction and development of potato microtubers under in vitro condition were investigated.
以两个马铃薯普通栽培种“Atlantic”和“甘农薯2号”的无毒试管苗为材料,研究了离体条件下,GA3和IAA对匍匐茎发生和结薯的影响,及在此条件下固体培养、固液双层培养和液体培养3种不同培养方式对马铃薯微型薯诱导和发育的影响。
2)  NLN solid liquid double medium
NLN固液双层培养基
3)  Liquid-solid culture
液固双相培养
1.
Using the Liquid-solid culture medium to select the suitable medium culture for the strain of Bbr84 and determine the spore yield of different medium,different time and different illumination of development.
结果表明:用液固双相培养法培养布氏白僵菌获取高孢粉,在液相培养阶段,SDAY+2%麦芽糖是较好的培养基;固相培养阶段,以大米+稻壳+黄粉虫粪组合产孢量较高,且在培养的第五天白僵菌产孢量即达到高峰,这比已报道的其他培养料配方的产孢高峰时间缩短2-3天。
4)  Solid-liquid medium
固-液培养基
5)  two phase culture
双液相培养
6)  Anther culture with solid medium and liquid medium in succession
双态双层培养法
补充资料:Earle培养液或营养液


Earle培养液或营养液


该液即EBSS,Earle’s平衡盐溶液(balanced salt solution),含氯化钙(CaCl2)(无水)1.8mM,氯化钾(KCl)5.3mM,硫酸镁(MgSO4)(无水)0.8mM,氯化钠(NaCl)117.0mM,碳酸氢钠(NaHCO3)26.0mM,磷酸钠(NaH2PO4·H2O)1.0mM,D-葡萄糖(Dglucase)5.6mM,酚红(phenol red)0.03mM,液体1000ml。
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