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1)  α-toxin gene
α-毒素基因
1.
Cloning and expression of α-toxin gene of Clostridium welchii isolated from Guizhou;
魏氏梭菌贵州分离株α-毒素基因的克隆与表达
2)  alpha-toxin gene
α毒素基因
1.
95 kb gene fragment from plasmid pETXA1 containing Clostridium perfringens type C alpha-toxin gene was amplified by PCR and it was inserted into recombinant plasmid pETXB1B2 containing 1.
为了构建具有良好免疫原性的α-β1-β2融合蛋白,利用PCR技术,从含C型产气荚膜梭菌α毒素基因的克隆质粒pETXA1中扩增出0。
2.
Alpha-toxin gene was amplified from chromosomal DNA of Clostridium perfringens type C by polymerase chain reaction(PCR).
2kb的α毒素基因,将纯化的PCR产物与载体pGEM-T连接,转化至受体菌JM109中,经NcoI/EcoRI和BamHI/EcoRI酶切鉴定及核苷酸序列测定证实,重组质粒pXCPA1中含有α毒素全基因。
3)  alpha toxin
α毒素基因
1.
The alpha toxin antigen gene of Clostridium perfringens was expressed in recombinant strain BL21(DE3)(pXETA1) and BL21(DE3)plysS(pXETA1), and the expressed alpha toxin was recognized by anti sera of Clostridium perfringens type A with Western blot analysis.
对含有产气荚膜梭菌(Clostridiumperfringens)α毒素基因的重组菌株BL21(DE3)(pXETA1)和BL21(DE3)plysS(pXETA1),通过培养性状观察和小鼠接种试验,证明这2株重组菌株均无致病性。
2.
According to the alpha toxin gene sequences of Clostridium welchii published on Gene Bank and publications, a pair of primes were designed to amplify a 325bp specific alpha toxin fragments, A kind of survey method for Clostridium welchii with PCR was established.
根据GenBank上发表的α毒素基因序列,参照公开发表物设计了一对引物,扩增出1条325bp的特异性扩增条带,建立了魏氏梭菌α毒素基因聚合酶链式反应的检测方法。
4)  α-toxin gene probe
α-毒素基因探针
5)  toxin gene
毒素基因
1.
Toxin genes, including tst and sec genes, were amplified by using multiple PCR and the relationship between the presence of the two genes and fever was analyzed.
方法 收集创伤病房近 3年 SA培养阳性病例 2 9例 ,采用多重 PCR扩增 SA的毒素基因 tst和 sec,分析毒素基因的存在与发热的关系 ;通过 PCR扩增 MRSA基因组稀有限制区旁的 DNA序列 ,对 MRSA进行基因分型。
6)  Toxin genes
毒素基因
1.
Methods:Toxin genes were amplified by PCR, and detected by electrophoresis.
目的:建立金黄色葡萄球菌肠毒素基因的检测方法。
2.
coli O157 from men and animals and single toxin gene is not the sole characteristic,quintic and quartic PCR assay was developed for detecting the O antigen,H7 flagella antigen and four toxin genes in the same PCR system.
针对 O1 5 7∶H7大肠杆菌 O抗原抗血清同多种细菌有交叉反应 ,而单一毒素基因并不是 O1 5 7∶ H7所独有这一特点 ,建立了五重和四重 PCR方法 ,同时检测大肠杆菌 O1 5 7∶ H7的 O抗原、H抗原和 4种毒素基因 ,可在较短的时间内检测、鉴定大肠杆菌 O1 5 7∶ H7,并有较高的特异性 ,解决了传统细菌检测方法耗时长、灵敏度低 ,并有交叉反应等问
3.
A rapid differential diagnosis for toxin genes of pathogenic Escherichia coli isolates, based on polymerase chain reaction (PCR) technique, was developed to amplify the specific genes fragments: STa, STb, LT and SLT-2e.
这表明多重PCR可为猪源大肠杆菌毒素基因的检测提供一种更加快速、经济、易行的技术。
补充资料:α,α,α,α',α',α'-六氯对二甲苯
分子式:C8H4Cl6
分子量:312.84
CAS号:68-36-0

性质:白色针状或粉末状结晶。熔点108-110℃。溶于二甲苯、石油醚、乙醇、植物油,不溶于水。无味,有特殊臭味,遇光、碱会缓慢分解而呈酸性。

制备方法:以混二甲苯为原料,先用98%硫酸磺化,使间二甲苯生成间二甲苯磺酸盐。从磺化反应物中分离出含邻、对二甲苯的油层,水洗、干燥,减压蒸馏出邻、对二甲苯。间二甲苯磺酸盐经水解可得副产品间二甲苯。由邻、对二甲苯经氯化即得1,4-双(三氯甲基)苯:在反应锅中投入邻、对二甲苯,再加入过氧化苯甲酰和三乙醇胺。加热到70℃后,在光照射下导入氯气,于70-80℃反应6h,再升温至100-120℃继续反应,至反应液相对密度达到1.560-1.580(65℃),即为反应终点,停止通氯,减压脱除余氯。降温至5℃,过滤,洗涤得粗品,重结晶,活性炭脱色得成品。

用途:抗血吸虫病药物。对肝吸虫病、阿米巴原虫病、疟疾以及肠道线虫有一定疗效。但对神经系统的不良反应较多见,且延迟反应持续较久。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。
参考词条