1) Basic phospholipase A 2
碱性磷脂酶A2
1.
The gene of the basic phospholipase A 2 from Agkistrodon halys Pallas (BPLA 2 )was mutated site-directedly by polymerase chain reaction (PCR) and the residue Arg 34 of the encloding protein was substituted by Glu and Gln, respectively.
用聚合酶链式反应 (PCR)对江浙蝮蛇毒碱性磷脂酶A2 基因进行点突变 ,使对应 34位氨基酸残基由Arg分别突变为Glu和Gln ,将其基因克隆至温敏表达载体pBLMVL2 ,并在大肠杆菌RR1中成功诱导表达 ,表达产物以包涵体的形式存在。
2) anti-phospholipase A 2 activity
抗磷脂酶A2活性
3) APLA2
酸性磷脂酶A2
1.
Objective To construct recombinant plasmids encoding acidic phospholipase A2-1(APLA2-1) from Guangxi Ophiophagus hannah(King cantor) Venom and express in E.
目的构建广西眼镜王蛇毒酸性磷脂酶A2-1(APLA2-1)在不同载体的重组表达质粒,在E。
2.
A cDNA encoding acidic phospholipase A2-1(APLA2-1) From Guangxi Ophiophagus Hannah(King Cobra) Venom was inserted into bacterial expression vector pBLMVL2, pET28a(+) and expressed in E.
将广西眼镜王蛇毒酸性磷脂酶A2-1(APLA2-1)基因克隆至表达载体pBLMVL2和 pET28a(+),分别转化入大肠杆菌RR1和BL21,经过诱导,SDS-PAGE检测,重组质粒pBLMVL2-APLA2-1无明显表达, pET28a-APLA2-1在18kD有一明显的表达条带,表达产物APLA2-1约占细菌总量30%,并以包涵体的形式存在,将产物变复性后用Sephadex G-75 纯化,产物经过SDS-PAGE检测只有单一条带, 通过Western-blot检测,证实纯化产物即是酸性磷脂酶A2 ，最后对表达的APLA2进行了酶活性和生物活性的测定，至此我们在大肠杆菌中表达了广西眼镜王蛇毒APLA2 。
4) phospholipase A2
磷脂酶A2
1.
Detection and Clinical Significance of Phospholipase A2 in Semen of Male Infertile Patients;
男性不育病人精液中磷脂酶A2的测定及其临床意义
2.
Construction and Identification for Cell Strain of Anti-human Seminal Plasma Phospholipase A2 Monoclonal Antibody;
抗人精浆磷脂酶A2单克隆抗体细胞株的建立与鉴定
3.
The Study of Phospholipase A2 Activity in Cerebrospinal Fluid of Intracranial in Fected Patients;
颅内感染患者脑脊液中磷脂酶A2活性的研究
5) phospholipase A_2
磷脂酶A2
1.
The phospholipase, phospholipase A_2 activity and meat quality traits from normal pork and PSE pork were determined.
宰后PSE肉的pH1h和pH24h低于正常肉,而L值(宰后1h、24h)、脂肪氧化程度、汁液流失率、贮藏损失、蒸煮损失和磷脂酶A2总活性明显高于正常肉。
2.
The hydrolysis of lecithin by phospholipase A_2 was firstly studied in vesicle solution and the influence of relevant parameters was investigated.
首次在泡囊水溶液中研究磷脂酶A2 催化水解卵磷脂并考察了各种因素对该反应的影响。
3.
Objective: To improve a method for the determination of the phospholipase A_2 activity,the effects of glycyrrhizin and platycodin on phospholipase A_2 activity in vitro are observed.
目的:建立一种体外磷脂酶A2活性测定的方法,并观察甘草总皂苷、桔梗总皂苷体外对磷脂酶A2活性的影响。
6) phospholipase A 2
磷脂酶A2
1.
To attain the optimal technological conditions of enzymic by drolysis of soybean phospholipid,the concentrated soybean phospholipid was selected as substrate, the phospholipase A 2 as the hydrolysis tool enzyme,and the enzyme-modified phospholipid was studied by the means of quiescence reaction.
为获取制备酶水解磷脂的最佳条件 ,以浓缩磷脂为底物 ,磷脂酶A2 为水解酶 ,采用静止反应方式 ,进行了大豆磷脂酶水解改性研究。
2.
objective: To study the pathogenesis of acute lung injury (ALI) of rabbits induced by intravascular injection of endotoxin (ET), as well as the role of the activation of phospholipase A 2(PLA 2).
目的 :研究大肠杆菌内毒素致兔急性肺损伤 (ALI)发病机制及磷脂酶A2 (PLA2 )激活在此病理生理过程中的致伤机制。
3.
Objective: To investigate the changes of levels of tumour necrosis α(TNF α), prostaglandin E 2 (PGE 2 ), and phospholipase A 2(PLA 2)in cerebrospinal fluid of patients with tuberculous meningitis and their clinical significances.
目的 :探讨肿瘤坏死因子 α(TNF α) ,磷脂酶A2 (PLA2 ) ,前列腺素E2 (PGE2 )在结核性脑膜炎患者脑脊液中的变化及临床意义。
补充资料：蛋磷脂 ,卵磷脂
药物名称：卵磷脂
英文名：Lecithin
别名： 胆碱磷酯;蛋黄素;蛋磷脂 ,卵磷脂
适应症： 用于防治动脉粥样硬化、脂肪肝、高胆固醇血症、急慢性肝炎、肝硬变、神经衰弱、消瘦及贫血等。
用量用法： 口服：每次０．６ｇ，每日３次。
规格： 片剂：０．１ｇ。
类别：调节血脂及抗动脉硬化药||肠内、肠外营养药
英文名：Lecithin
别名： 胆碱磷酯;蛋黄素;蛋磷脂 ,卵磷脂
适应症： 用于防治动脉粥样硬化、脂肪肝、高胆固醇血症、急慢性肝炎、肝硬变、神经衰弱、消瘦及贫血等。
用量用法： 口服：每次０．６ｇ，每日３次。
规格： 片剂：０．１ｇ。
类别：调节血脂及抗动脉硬化药||肠内、肠外营养药
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条