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1)  Yeast twohybrid system
酵母双杂合体系统
2)  yeast two hybrid system
酵母双杂合系统
1.
Objective To construct the expression plasmid pGBT9 hERR1/HBD as a bait in yeast two hybrid system.
目的 构建酵母双杂合系统诱饵蛋白表达质粒pGBT9 hERR1 HBD。
2.
To find downstream signal transducer of Jak3, the N terminal region JH3-JH7 of Jak3 was used as a bait to screen a cDNA library by using the yeast two hybrid system.
为了寻找它的下游分子,利用酵母双杂合系统以Jak3的N端JH3~JH7区域筛选cDNA库。
3.
The yeast two hybrid system has been used to screen the proteins interacting with c Mpl.
利用酵母双杂合系统(tw o-hybrid system )筛选与c-Mpl相互作用的蛋白质因子,以Gal4 BD融合c-Mpl膜内部分cDNA 的pASMM 为靶蛋白质粒,筛选了人胎盘cDNA 文库,分离到人波形纤维蛋白(vim entin)的部分编码序列,首次检测到波形纤维蛋白与TPO 受体之间的相互作用,这提示细胞骨架蛋白可能在TPO 的信号转导过程中起着重要的作
3)  yeast two-Hybrid system
酵母双杂合体系
1.
Objective: This study was designed to seek the hepatitis B virus preSl associated protein from normal human liver cDNA library by yeast two-Hybrid system and explore the role of preSl gene in the infection of HBV.
目的:利用酵母双杂合体系筛选乙型肝炎病毒前S1(preS1)蛋白相关蛋白,以利于探讨前S1蛋白在乙型肝炎病毒的入胞机制。
4)  Yeast two-hybrid system
酵母双杂交系统
1.
Screening for protein interacting with FGFR3 in mouse 17.5-day embryo cDNA library by yeast two-hybrid system;
应用酵母双杂交系统筛选FGFR3相互作用的蛋白质
2.
Application of yeast two-hybrid system to plant virology;
酵母双杂交系统在植物病毒学上的应用
3.
To screen for proteins interacting with collectrin, the yeast two-hybrid system was performed.
通过酵母双杂交系统从人肾脏cDNA文库中筛选与collectrin相互作用的蛋白 ,可以为该基因的功能研究提供线索 。
5)  Yeast Two Hybrid System
酵母双杂交系统
1.
Recombination of bait vector pGBKT7-Hsp70 and the test of its autonomous activation in the yeast two hybrid system;
HSP70诱饵载体的构建及其在酵母双杂交系统中自激活作用的检测
2.
The interaction between CD14 and TLR4 was identified by yeast two hybrid system and glutathione S transferase (GST) pull down test and coimmunoprecipitation assay.
酵母双杂交系统检测显示pGADT7AD CD14和pGBKT7BD TLR4质粒均无自主激活报告基因LacZ表达的能力 ,在酵母细胞中CD14和TLR4之间存在相互作用。
6)  yeast two-hybrid system
酵母双杂交体系
1.
A ‘bait’ vector of yeast two-hybrid system that clearly expressed IBV BM-2 protein in yeast cell and ruled out of its self-activation was constructed using Open Reading Frame (ORF).
用B型流行性感冒病毒BM2 蛋白开放阅读框架片段 ,构建酵母双杂交体系中的“饵”载体 ,明确其在酵母细胞中的表达 ,排除自身激活作用 ,并用酵母双杂交法从人cDNA基因库中筛选出与BM2 蛋白相互作用的蛋白。
2.
Objective To gain an insight into the function of FXR1P and guess the relationship between FXR1P and the pathogenesis of fragile X syndrome, yeast two-hybrid system was used to screen proteins interacting with FXR1P.
目的 应用酵母双杂交体系筛选人胎脑cDNA文库中与脆性X智力低下相关蛋白1(FXR1P)相互作用的蛋白质,进而了解FXR1P的功能,推测FXR1P与脆性X综合征发病机制的关系。
补充资料:杂合体
分子式:
CAS号:

性质:又称杂合体,杂种。由遗传学上不同的种或个体之间杂交而产生的生物。种间杂交可能是不育的,因为在减数分裂时,不相同的染色体之间不能配对。F1杂种的培育是为了利用其杂交优势及高产性,对植物尤其是如此。

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