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1)  gene targeting by temporally and spatially
时空基因打靶
2)  Gene targeting
基因打靶
1.
Construction of LRP16 gene targeting vector;
小鼠LRP16基因打靶载体的构建
2.
Research on gene targeting to multiple loci in cow fetal fibroblasts;
奶牛胎儿细胞多位点基因打靶的研究
3.
Research of multiple locus gene targeting in vivo of Siniperca;
多位点基因打靶技术的鳜鱼体内实验研究
3)  gene targeting
基因打靶法
4)  Gene targeting
基因打靶技术
1.
In the method of gene targeting, exogenous gene fragment could be inserted in genome with homologous recombinase, which could change the gene locus exactly in the producing of transgenic animals and solve a series of problems with random insertion in the other methods.
基因打靶技术因其通过同源重组的方法整合进基因组,在转基因动物生产中可以定点的改造动物的遗传基因,克服了其它转基因方法中随机插入而引起的一系列问题。
5)  gene targeting vectors
基因打靶载体
1.
To generate a loxPneoloxP selection cassette for construction of goat mammary gland gene targeting vectors, loxP sites were introduced into both the forward and reverse primers and a plasmid pTarget containing neo gene was used as the template for high fidelity PCR amplification.
为构建山羊乳腺基因打靶载体的选择标记盒,将loxP位点分别引入正、反向引物,以含新霉素磷酸转移酶(neo)基因的质粒pTarget为模板,用高保真聚合酶链反应(PCR)扩增得loxP neo loxP选择标记盒。
6)  gene targeting in somatic cell
体细胞基因打靶
补充资料:基因打靶法
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性质:又称同源重组技术(homelogens DNA technology)。是转基因方法的一种。利用一适当的DNA可在靶位相结合,使人们可在染色体的某个特定部位剔除或插进一个基因。可用来修饰一个现存基因,在器官移植中有用,还可用来发展某些人体遗传病的动物模型。

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