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1)  tobacco suspension cells
烟草悬浮培养细胞
1.
Mechanical stimulation(MS)induced H_2O_2 accumulation in tobacco suspension culture cells,in- creased survival percentage and regrowth of tobacco suspension cells under high temperature at 43℃,and alleviated the loss of cell vitality and membrane damage.
机械刺激可诱发烟草悬浮培养细胞中H_2O_2的积累,提高烟草悬浮培养细胞在高温胁迫下的存活率和再生能力,缓解高温胁迫下的细胞活力丧失和膜伤害,外源H_2O_2预处理也可提高烟草悬浮细胞的耐热性。
2.
In addition,the pretreatments with peroxidase inhibitors,KCN or salicylhydroxamic acid (SHAM),respectively,inhibited sharply MS-induced H2O2 burst in tobacco suspension cells and the increase of pH value in culture medium.
机械刺激可提高烟草悬浮培养细胞中CWPOD的活性,促进烟草悬浮培养细胞中H2O2的积累,增加悬浮细胞培养介质的pH值。
2)  Glycyrrihizic suspension culture cell
甘草悬浮培养细胞
1.
By different extractant,glycyrrihizic acid can be extracted from glycyrrihizic roots,stems,leaves,herbal medicine and Glycyrrihizic suspension culture cells.
采用不同的萃取剂从甘草的根、茎、叶、草药和甘草悬浮培养细胞中萃取甘草酸。
3)  cell suspension cultures of Glycyrrhiza inflata
悬浮培养甘草细胞
4)  tobacco suspension culture cell
烟草悬浮培养
5)  tobacco BY-2 suspension cell
烟草BY-2悬浮细胞
1.
0μg/mL microcystin-RR (MC-RR) to treat tobacco BY-2 suspension cells, the cell viability, contents of protein, soluble sugar, nitrate-nitrogen, and total phosphorus in cells were determineded; and the viability change condition of acid phosphatase (ACP) was detected.
0μg/mL的微囊藻毒素-RR(MC-RR)处理烟草BY-2悬浮细胞,测定了细胞活力、细胞内蛋白质含量、可溶性糖含量、硝态氮含量及总磷含量,并且检测了酸性磷酸酶(ACP)的活力变化情况。
6)  tobacco suspension cells
烟草悬浮细胞
1.
Isolation of prevacuolar compartments from tobacco suspension cells;
从烟草悬浮细胞中分离液泡前体的方法研究
2.
In this study,we applied different concentrations of oligochitosan to tobacco suspension cells to investigate the change in gene expression involved in jasmonic acid biosynthesis.
采用RT-PCR方法研究了不同浓度壳寡糖对烟草悬浮细胞茉莉酸合成酶基因的转录调控。
补充资料:植物细胞悬浮培养
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性质:植物细胞或小的细胞聚集体在液体培养基中(于摇床上)进行悬浮培养,这些细胞和小的聚集体来自愈伤组织、某个器官或组织、甚至幼嫩的植株,通过物理或化学的方法进行分离而获得。一个成功的悬浮细胞培养体系必须满足三个基本条件:一是悬浮培养物分散性良好、细胞团较小,二是均一性好,三是生长迅速,悬浮细胞的量一般2~3天甚至更短时间可增加一倍。

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