1) high-density cultivation
黑曲霉菊粉内切酶
1.
Objective: To optimize the expression conditions of endo-inulinase from Aspergillus niger in high-density cultivation of recombinant Pichia methanolica inu-26.
目的:对毕赤嗜甲醇酵母工程菌inu-26高密度培养表达黑曲霉菊粉内切酶的条件进行优化,找出最佳的外源蛋白表达条件。
2) endoinulinase
内切菊粉酶
1.
Purification and Properties of Endoinulinase from Chaetomium sp.;
毛壳霉内切菊粉酶的纯化与性质
2.
Three of them were identified as exoinulinase,and the rest one mainly contained endoinulinase.
采用硫酸铵分级沉淀、透析脱盐、DEAE-Cellulose离子交换色谱等分离纯化技术,从As-pergillus ficuum菌株混合酶系中分离得到4种菊粉酶组分,应用薄层层析法分离各组分水解菊粉的产物,发现其中3种组分主要含外切菊粉酶,一种主要含有内切菊粉酶。
3.
Proved using TLC and HPLC, three bands had exoinulinase activity and two bands had endoinulinase activity.
采用活性聚丙烯酰胺凝胶电泳法对Aspergillusficuum产果聚糖酶体系进行了分离 ,获得8条谱带 ;进一步运用薄层色谱 (TLC)和高效液相色谱 (HPLC)法进行分析 ,发现 8条谱带中有 3条属于外切菊粉酶 ,2条属于内切菊粉酶 ,证明了Aspergillusficuum能同时产内切菊粉酶和外切菊粉酶 。
3) endo-inulinase
菊粉内切酶
1.
Objective:To produce the inulo-oligisaccarides from chicory inulin by using recombined endo-inulinase of Aspergillus niger.
目的:利用毕赤嗜甲醇酵母表达的重组黑曲霉菊粉内切酶,从菊苣菊粉中制取低聚果糖。
2.
Using endo-inulinase from microorganism to produce inulo-oligosaccharides has become the focus of these studies, but most endo-inulinase from the natural microorganism is exo-inulinse or the mixtures of endo-inulinase and exo-inulinase.
近年在开发菊粉系列产品时是以菊粉寡糖这一功能成分为研究目标的，利用微生物菊粉内切酶进行菊粉寡糖的生产成为现在开发菊粉系列产品的研究热点，但大多天然微生物分泌的菊粉酶为外切酶或外切酶和内切酶的混合物，只有少数微生物产菊粉内切酶，并且其含量甚微、从而使分离纯化困难，不利于工业化生产。
4) exoinulinase
外切菊粉酶
1.
The expression of exoinulinase from Kluyveromyces marxianus in Pichia pastoris;
克鲁维酵母中外切菊粉酶基因在毕赤酵母中的表达研究
2.
Three of them were identified as exoinulinase,and the rest one mainly contained endoinulinase.
采用硫酸铵分级沉淀、透析脱盐、DEAE-Cellulose离子交换色谱等分离纯化技术,从As-pergillus ficuum菌株混合酶系中分离得到4种菊粉酶组分,应用薄层层析法分离各组分水解菊粉的产物,发现其中3种组分主要含外切菊粉酶,一种主要含有内切菊粉酶。
3.
Proved using TLC and HPLC, three bands had exoinulinase activity and two bands had endoinulinase activity.
采用活性聚丙烯酰胺凝胶电泳法对Aspergillusficuum产果聚糖酶体系进行了分离 ,获得8条谱带 ;进一步运用薄层色谱 (TLC)和高效液相色谱 (HPLC)法进行分析 ,发现 8条谱带中有 3条属于外切菊粉酶 ,2条属于内切菊粉酶 ,证明了Aspergillusficuum能同时产内切菊粉酶和外切菊粉酶 。
补充资料：菊粉
分子式：
CAS号：
性质：见菊糖
CAS号：
性质：见菊糖
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条