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1)  isothermal DNAamplifica-tion
等温DNA扩增
2)  LAMP [英][læmp]  [美][læmp]
等温扩增
1.
A novel nucleic acid amplification method,termed loop-mediated isothermal amplification(LAMP),which amplifies DNA with high specificity,efficiency,and rapidity under isothermal conditions,may be a valuable tool for the rapid detection of infectious diseases.
环介导等温扩增(loop-mediated isothermal amplification,简称LAMP)是利用4个特殊设计的引物和具有链置换活性的DNA聚合酶,在恒温条件下特异、高效、快速地扩增DNA的新技术。
2.
A novel nucleic acid amplification method, termed loop-mediated isothermalamplification (LAMP), which amplifies DNA with high specificity, efficiency, andrapidity under isothermal conditions, may be a valuable tool for the rapid detection ofinfectious diseases.
环介导等温扩增(loop-mediated isothermal amplification,简称LAMP)是利用4个特殊设计的引物和具有链置换活性的DNA聚合酶,在恒温条件下特异、高效、快速地扩增DNA的新技术。
3)  loop-mediated isothermal amplification of DNA method
DNA介导恒温核酸扩增法
4)  DNA loop-mediated isothermal amplification
DNA环介导的恒温扩增法
5)  Loop-mediated isothermal amplification
环介导等温扩增
1.
Loop-mediated isothermal amplification method for the diagnosis of infectious diseases;
环介导等温扩增技术及在传染性疾病检测中的应用
2.
Optimal DNA amplification of lab-on-a-chip for loop-mediated isothermal amplification
环介导等温扩增芯片核酸最佳扩增条件研究
3.
Development of a loop-mediated isothermal amplification assay for rapid detection of Enterobacter sakazakii in powdered infant formula
改良环介导等温扩增技术快速检测婴儿配方奶粉中的阪崎肠杆菌
6)  LAMP [英][læmp]  [美][læmp]
环介导等温扩增
1.
To develop a loop-mediated isothermal amplification(LAMP)assay for the rapid and sensitive detection of Salmonella.
建立一种检测沙门菌属快速敏感的环介导等温扩增(loop-mediated isothermal amplification,LAMP)检测方法。
2.
Methods: The reaction system of detection of Toxoplasma gondii DNA was set up by loop-mediated isothermal amplification (LAMP) technology, and the reaction system was optimized, the specificity and sensitivity were evaluated.
方法:采用环介导等温扩增(loop-mediated isothermal amplification,LAMP)技术建立了检测弓形虫DNA的方法,并进行特异性、敏感性评估,在此基础上建立依据SYBR Green I的定量方法及通过显色反应直接判断结果的简便方法。
3.
Methods:We designed two specific LAMP(Loop-mediated isothermal amplification) primers for HSV-Ⅰand HSV-Ⅱrespectively,and established the reaction system to detect HSV by LAMP.
方法:分别设计了HSV-Ⅰ和HSV-Ⅱ型特异的环介导等温扩增(Loop-mediatedisothermal amplification LAMP)引物,并利用该技术建立了快速检测HSV DNA的反应体系。
补充资料:随机扩增多态DNA技术
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性质:这一技术是1990年由威兰斯(Willams)和韦尔什(Welsh)两个研究小组几乎同时建立和发展起来的一种新型遗传标记技术。具有检出DNA多态性的方式,同时还具有快速、简捷、高效等优点。它能从大量杂交后代中鉴别出真杂交种,和从原生质体融合后的真融合子。它提供了比同功酶更为准确的杂种及融合子鉴定方法,从而获得了确切的杂交和原生质融合的分子生物学证据。另外还可利用RAPD技术进行基因组指纹图谱构建、遗传连锁图构建、和品种及亲缘关系的鉴别等。

说明:补充资料仅用于学习参考,请勿用于其它任何用途。
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