1) truncated cDNA ends
截短cDNA末端
2) 5'-cDNA end
5-'cDNA末端
1.
The 5′-cDNA ends of Na+/H+ antiporter gene on the tonoplast of Aeluropus littoralis were cloned by RLM-RACE and the transcription initiation site was detected.
sinensisDebeaux)液泡膜Na+/H+逆向转运蛋白(Na+/H+antiporter)基因部分cDNA片段,设计2条特异引物(GSP1、GSP2),采用RLM-RACE(RNA ligase-medi-ated rapid amplification of 5′and 3′cDNA ends)法获得了其5-′cDNA末端序列,并找出了转录起始位点。
3) 3'cDNA end
3'cDNA末端
4) 5'cDNA end
5'cDNA末端
5) RACE
[英][reɪs] [美][res]
cDNA末端扩增
1.
Gene specific primers derived from this partial sequence were used for the amplification of the 3′- and 5′-ends of this cDNA by RACE method,and this lead to a full-length cDNA sequence of 1 482 bp was amplified.
根据真菌Δ6 脂肪酸脱氢酶保守的氨基酸序列设计简并引物进行RT PCR ,获得一个 5 93bp的cDNA片段 ,再根据获得的部分序列设计基因特异性引物 ,通过cDNA末端扩增技术 (RACE)获得该cDNA的 3′和 5′序列 ,从而得到全长为 14 82bp的cDNA序列。
2.
A lysozyme gene from the Chinese oak silkworm,Antheraea pernyi,was cloned by RACE using degenerate primers designed from several conserved amino acid regions shared among related insect species.
采用cDNA末端扩增(RACE)方法及简并引物克隆了柞蚕溶菌酶(Antheraea pernyilysozyme)基因(Gen-Bank登录号:DQ353869)。
6) RACE
[英][reɪs] [美][res]
快速cDNA末端扩增
1.
The rapid amplification of cDNA ends(RACE)technique and the electronic cDNA library select mathod was applied to amplify full length of the expressing sequence tag(EST) ,which is a specfic-stage in Schistosoma japonicum adult worm, and a cDNA fragment with an ORF was identified(GenBank accession number is AY847290).
利用快速cDNA末端扩增(RACE)技术结合“电子cDNA文库”筛选法,对日本血吸虫成虫期特异表达基因EST片段的全长序列进行了扩增,获得了一含完整开放性阅读框(ORF)的基因序列(GenBank中的登录号为AY847290)。
补充资料：断截截
1.形容剪裁利索。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条