Genetic Analysis of a Broad-spectrum Blast Resistance Gene d12 and its Employment in Marker-assisted Selection;

Optimal RAPD-PCR conditions developing and molecular identification marker selecting for Bursaphelenchus xylophilus;

In order to develop the optimal RAPD-PCR system for selecting molecular identification markers of B.

With the optimized system,amplified bands were clear and stable without disturbed background,which would provide a powerful tool for building the AFLP fingerprint of Gastrodin and molecular marker-assisted breeding.

The affecting factors and developing prospect of marker-assisted selection;

This project used the strategy of discarding susceptible individual Plants and keeping resistance ones by resistance identification in breeding populations and Planted resistant plants next in earlier generations (F1 - F3), and performing marker-assisted selection combining resistance identification in later generation (F4 generation) for pyramiding powdery mildew resistance.

In order to improve the precision of marker-assisted selection for fragrance gene in rice, a functional marker GRFM04 has been developed based on the deletion of 8 bp in DNA sequence of BAD2 gene, namely the fragrance gene, in the fragrant rice varieties.