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1)  HSV1-TK gene
HSV1-TK基因
1.
Objective:To construct the prokaryotic expression plasmid included HSV1-TK gene and detect the result of expression,the basis for using bacteria vector in tumor gene therapy was made in this work.
目的:构建含有HSV1-TK基因的原核表达质粒,并对其原核表达情况进行检测,为进一步将细菌载体应用于肿瘤基因治疗奠定基础。
2.
Firstly,plasmid pHSV-106 was taken as the template,the clone of HSV1-TK gene was acquired through PCR and connected to the prokaryotic expression vector pGEX-6P-1,then recombinant plasmid was identified and transformed into E.
首先以质粒pHSV-106为模板,通过PCR获得HSV1-TK基因克隆并将其与原核表达载体pGEX-6P-1连接,重组质粒经鉴定后将其转化到E。
2)  HSV_1-tk/GCV
HSV1-tk/GCV
1.
Objective To study whether Topotecan could enhance the killing effect of a suicide gene therapy sy-stem of HSV_1-tk/GCV (herpes simplex ivirus thymidine kinase / ganciclovir ) for ovarian cancer in vivo.
目的探讨Topotecan能否增强HSV1-tk/GCV自杀基因系统对卵巢癌的体内治疗作用。
3)  HSV1-TK/GCV system
HSV1-TK/GCV系统
4)  HSV-TK gene
HSV-TK基因
1.
Lung carcinoma targeted expression of HSV-TK gene controlled by radioinducible synchronizing positive feedback genetic circuit;
放射诱导HSV-TK基因在肺癌细胞中靶向、高效性表达的研究
2.
MethodsHuman Tenon s capsule fibroblasts transferred by HSV-tk gene(HTFs-tk) and normal HTFs cells were mixed at a different ratio and seeded at two cell densities,and these cells were exposed to 5 μg/ml ganciclovir.
方法将转HSV-tk基因HTFs(HTFs-tk)与正常HTFs于两种接种密度下以不同比例混合,加入5μg/m l更昔洛韦作用5 d,MTT法检测对HTFs的杀伤作用;染料传输法观察HTFs细胞间隙连接细胞间通讯(G JIC)的能力;免疫细胞化学检测Cx43分布;RT-PCR及W estern b lot检测Cx43表达。
3.
Single clone harbouring HSV-TK gene was picked after G418 selection.
用G418筛选到稳定表达HSV-TK基因的细胞克隆LoVo/LTKSN。
5)  tk gene
tk基因
1.
Study on Construction of the homologous recombinant adenovirus tk gene;
腺病毒-tk基因同源重组系统构建的研究
2.
Construction and identification of recombinant adenovirus with rPB-DR promoter and TK gene;
含rPB-DR启动子和TK基因的重组腺病毒的构建和鉴定
3.
Relation between immune response and bystander effect when using tk gene to treat therapy glioma;
tk基因治疗脑胶质瘤时的免疫反应与旁观者效应的关系
6)  HSV tk gene
HSV-tk基因
补充资料:HSV
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性质:疱疹病毒中的一种,可以感染人及各种动物。

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