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1)  three T-DNAs
三段T-DNA
2)  T-DNA insertion
T-DNA插入
1.
Effection of pigments and the protein complexes of thylakoid membrane in Hcf-1 mutant of Arabidopsis by T-DNA insertion;
T-DNA插入对拟南芥突变体色素和内囊体膜色素蛋白复合物的影响
2.
A disease lesion mimic mutant named AZT91 was screened from T-DNA insertion mutant of rice.
从T-DNA插入突变体中筛选到一个类病斑突变体AZT91,主要表现为生长缓慢、植株矮小、叶片出现条状褐斑,最后死亡。
3.
In this study,an albino mutant caused by T-DNA insertion in rice was identified.
Basta抗性检测、PCR分子检测及Southern杂交证实,该突变体是由单一T-DNA插入所引起的,白化苗性状与T-DNA共分离。
3)  T-DNA tag
T-DNA标签
1.
Using calli from cotyledon and hypocotyl of pepper inbred line H8124 as explants,T-DNA tag was inserted into pepper through agrobacterium-mediated transformation.
以辣椒自交系H8124无菌苗子叶和下胚轴为外植体,采用根癌农杆菌侵染转化法,将T-DNA标签导入辣椒,以探讨影响转化效率的主要因素,包括外源植物生长调节剂浓度、农杆菌类型、外植体材料、预培养时间、侵染时间等。
4)  T-DNA location
T-DNA定位
5)  T-DNA tagging
T-DNA标签
1.
T-DNA tagging and its applicatlon in plant functional genome research;
T-DNA标签技术及其在植物功能基因组研究中的应用
2.
The paper summarized the procedure for analyzing gene function by T-DNA tagging and the methods to clone the tagged gene of mutants gotten by transformation,which would facilitate the analysis of gene function using T-DNA tagging.
概述了应用T-DNA标签进行基因功能分析的步骤及获得突变体后标签基因的分离方法,对应用T-DNA标签进行基因功能分析研究具有一定的参考价值。
3.
Agrobacterium-mediated T-DNA tagging is an effective molecular technology in recent years.
农杆菌介导的T-DNA标签法是近年发展的一种有效的分子生物学技术。
6)  Double T-DNA
双T-DNA
1.
Construction and Verification of Double T-DNA Plant Expression Vector of the LFY Gene;
LFY基因双T-DNA植物表达载体的构建
2.
Construction and Verification of Double T-DNA Plant Expression Vector of the DREB Gene;
DREB基因双T-DNA植物表达载体的构建及验证
3.
A feasible approach to eliminate the selectable marker genes from transgenic plants is the use of a "double T-DNA" binary vector system.
双T-DNA系统是实现筛选完成后选择标记基因删除的一种简便可行的方式。
补充资料:DNA分子花窗(沿B―DNA轴线方向看)




DNA分子花窗(沿B―DNA轴线方向看)
  [图]图

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