1) RT-PCR
逆转录-多聚合酶链反应
1.
E-Cad mRNA was evaluated in the above mentioned samples by semi-quantitative RT-PCR,and the correlation between E.
方法采用免疫组织化学染色的方法观察比较E-Cad在进展期胃癌原发灶及转移淋巴结中的表达;同时用逆转录-多聚合酶链反应(RT-PCR)方法检测上述标本中E-Cad基因的表达,并对其和临床病理参数的关系进行分析。
2.
Methods: Reverse tanscriptase-polymerase chain reaction (RT-PCR) method was applied to detect the expression of HMGA1-mRNA and HMGA2-mRNA and Western blot analysis respectively was applied to detect the expression of HMGA1 and HMGA2 protein in endometrial carcinoma cell lines AN3CA, ECC-1, Ishikawa and tissue specimens from cacinomatous endometrium (n=21) and normal endometrium (n=18).
方法:采用逆转录-多聚合酶链反应、免疫印迹法检测HMGA1与HMGA2在体外培养的子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及21例子宫内膜癌组织标本(研究组)和18例正常子宫内膜组织标本(对照组)中的表达。
3.
Methods:The expressions of HMGA2-mRNA and HMGA2 protein were detected in endometrial carcinoma cell lines AN3CA,ECC-1,Ishikawa and tissue specimen from cacinomatous endometrium(n=21)and normal endometrium(n=18)using reverse tanscriptase-polymerase chain reaction(RT-PCR)and Western blot analysis,respectively.
方法:采用逆转录-多聚合酶链反应(RT-PCR)、免疫印迹法(Western Blot)检测HMGA2在体外培养的子宫内膜癌细胞系AN3CA、ECC-1、Ishikawa以及21例子宫内膜癌组织标本和18例正常子宫内膜组织标本中的表达。
2) reverse transcription polymerase chain reaction
逆转录多聚合酶链反应
3) Reverse transcription polymerase chain reaction
逆转录酶聚合酶链反应
4) Reverse transcriptase polymerase chain reaction
逆转录酶聚合酶链反应
6) RT-PCR
逆转录多聚酶链式反应
1.
Also RT-PCR was used to determine the expressions of STAT3 mRNA and Survivin mRNA.
方法采用免疫组织化学方法检测40例喉鳞状细胞癌组织和12例喉正常黏膜组织中STAT3、磷酸化STAT3(p-STAT3)蛋白和Survivin蛋白的表达;运用逆转录多聚酶链式反应(RT-PCR)检测上述组织中STAT3 mRNA和Survivin mRNA的表达。
2.
Methods: Total RNA was extracted from 37 cases of human gastric carcinoma tissues and the corresponding para-carcinoma tissues,and the levels of Wnt2B gene mRNA expression were evaluated by Reverse transcription-polymerase chain reaction (RT-PCR).
方法:采用逆转录多聚酶链式反应(RT-PCR)技术检测胃癌组织及相应癌旁组织中Wnt2B基因mRNA的表达水平。
3.
Objective:To found RT-PCR method to amplify HIV-1 RNA and apply the method to diagnose HIV-1 early infection.
目的 :建立 RT- PCR(逆转录多聚酶链式反应 )扩增 HIV核酸的检测方法并将其应用到 HIV感染的早期诊断中。
补充资料：聚合酶链反应
聚合酶链反应
polymerase chain reaction,PCR
是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA）片断大量增扩，达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体，因其假阳性率较高，故应谨慎使用。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条