1) Western-blot
Western斑点印迹法
1.
The HSP27/60/70 expression level in right atrial samples was determined by Western-blot,while the expression in serum samples was determined by enzyme-linked immunosorb.
方法选取瓣膜置换手术患者40例,其中20例为房颤持续时间超过36个月,另20例为窦性心律,以ELISA法检测血清HSP27/60/70含量,以Western斑点印迹法检测右心房心肌组织中HSP27/60/70的表达水平。
2.
The HSP27/60/70 level expression in right atrial samples was determined by Western-blot,the expression in serum samples was determined by ELISA.
所有患者手术均采用全麻体外循环,胸骨正中切口,体外循环开始前,取右心耳组织,立即置-70℃液氮保存,以Western斑点印迹法检测右心房心肌组织中HSP27/60/70含量,检测结果以积分光密度表示。
2) Western dot assay
Western斑点印迹分析
3) Western blotting
Western印迹法
1.
Using western blotting and MTT chemosensi-tive testing, the relationship between the expression of NFkB family proteins, P53 and MDM2 in ovarian cancer cell lines and chemotherapy resistance of ovarian cell was ob.
培养13个卵巢癌细胞系,提取癌细胞蛋白,采用Western印迹法观察NFkB家族蛋白、P53和MDM2的表达,并用四氮唑盐法药敏试验观察与卵巢癌细胞化疗耐药性关系。
2.
Using the mouse antibody to Hsp70of human,pig,dog,etc,Hsp70was detected from seven randomly sele cted yeast strains by Western blotting.
随机抽取其中7株新生隐球菌,采用鼠抗人、猪、狗等的Hsp70抗体,通过Western印迹法来检测其Hsp70,根据实验信号强弱定性判断在不同刺激条件下的Hsp70产量。
3.
Using Western blotting and MTT chemosensitive testing, the relationship between the expression of NFκB family proteins in ovarian cancer cell lines with chemotherapy resistance of ovarian cell was observed.
方法 :培养 13株卵巢癌细胞系 ,对其进行胞质、胞核蛋白质提取 ,Western印迹法观察NFκB家族蛋白及其抑制物的表达 ,并用MTT检测药敏、观察NFκB家族蛋白与卵巢癌细胞化疗耐药性关系。
4) Western blot
Western印迹法
1.
Methods Western blot and immunohistochemistry were performed to detect the expression and phosphorylation status of GluR1.
方法采用Western印迹法、免疫组织化学法检测纹状体AMPA受体亚单位GluR1表达及磷酸化状态。
5) Far-Western blotting
Far-Western印迹法
6) dot-blotting
斑点印迹法
1.
Objective To improve the purifying method of Jo-1 antigen from rabbit thymus used for detection of anti-Jo-1 antibody by dot-blotting immunoassay(DB).
目的改进从兔胸腺中纯化能用于斑点印迹法检测抗-Jo-1抗体、Jo-1抗原的方法。
补充资料：“诺森”印迹法
分子式：
CAS号：
性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。
CAS号：
性质：又称RNA印迹（法）。与DNA斑迹法过程类似的技术。用于从凝胶中转移出RNA片段转移至硝酸纤维素膜，尼龙膜等，并用探针进行杂交（称“北方”杂交）以显示目的区段的位置。显示RNA的大小及丰度。
说明：补充资料仅用于学习参考，请勿用于其它任何用途。
参考词条