Objective Take DNA sequencing method as the standard,to explore the sensitivity and specificity of HBV-YMDD mutation by PCR melting curve method.

Influence of experimental conditions on probe melting curves based on fluorescence resonance energy transfer;

Melting curve of target DNA fragments were drawn using real-time PCR through Light Cycler Instrument integrated with specific oligonucleotide probes,and the samples were genotyped according to the peak of the melting tempera.

Methods The HBV YMDD mutation was detected by fluorescent hybridization double probe PCR and melting curve assay(FH-PCR-MC) in 119 patients with CHB who were not treated with lamivudine and in 30 patients with CHB who were treated with lamivudine.

Rapid detection of GSTM1 polymorphisms using SYBR green I and melting curve analysis;

Detection of mtDNA A1555G mutation by allele-specific PCR and its melting curve analysis

Detection of RSV A and B were based on determination of specific melting temperatures by real-time PCR and melting curve analysis using SYBR GreenⅠfluorescent dye.

Here we will describe new methods such as denaturing high performance liquid chromatography(DHPLC),primer extension and time-of-flight mass spectrometry(TOF-MS),Tm-shift curve,allele-specific amplification-Tm shift,Molecular beacons and TaqMan.

High resolution melting analysis of small amplicons and of mixing with a known genotype for genotyping of mitochondrial SNP13928G > C

High resolution melting analysis which is based on the physical properties of nucleic acid for genotyping or mutation scanning of PCR products by using of high resolution instruments with special "saturation"dyes without special probes has recently been reported.